| Name: | Description: | Size: | Format: | |
|---|---|---|---|---|
| 588.18 KB | Adobe PDF |
Advisor(s)
Abstract(s)
The influence of geographic distribution and type
of habitat on the molecular epidemiology of ciprofloxacin
resistant Escherichia coli was investigated.
Ciprofloxacin resistant E. coli from wastewater,
urban water with faecal contamination and faeces of
gulls, pigeons and birds of prey, from Portugal,
Spain and Sweden were compared based on multilocus
sequence typing (MLST) and quinolone resistance
genetic determinants. Multi-locus sequence
typing allowed the differentiation of E. coli lineages
associated with birds of prey from those inhabiting
gulls and waters. E. coli lineages of clinical relevance,
such as the complex ST131, were detected
in wastewater, streams and gulls in Portugal, Spain
and Sweden.
Quinolone resistance was due to gyrA and parC
mutations, although distinct mutations were detected
in birds of prey and in wastewater, streams and gulls
isolates. These differences were correlated with specific
MLST lineages, suggesting resistance inheritance.
Among the plasmid-mediated quinolone
resistance genes, only aac(6′)-ib-cr and qnrS were
detected in wastewater, streams and gulls isolates,
but not in birds of prey. The horizontal transfer of the
gene aac(6′)-ib-cr could be inferred from its occurrence
in different MLST lineages
Description
Keywords
Pedagogical Context
Citation
VREDENBURG, Jana...[et al.] - Quinolone-resistant Escherichia coli isolated from birds of prey in Portugal are genetically distinct from those isolated from water environments and gulls in Portugal, Spain and Sweden. Environmental Microbiology. ISSN 1462-2920. Environmental Microbiology.Vol. 16 n.º 4 (2014), 995–1004
10.1111/1462-2920.12231
10.1111/1462-2920.12231
Publisher
Society for Applied Microbiology
Wiley
Wiley