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Functional and genomic characterization of Serratia quinivorans NFX21 and Pseudomonas thivervalensis NFX104, novel biocontrol agents against Botrytis cinerea

2026-04-01Artigo de investigação Acesso aberto
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dc.contributor.authorTedesco, Sara
dc.contributor.authorSilva, Filipa T.
dc.contributor.authorPimenta, Margarida
dc.contributor.authorGaspar, Frédéric Bustos
dc.contributor.authorSilva, Marta Nunes da
dc.contributor.authorCrespo, Maria Teresa Barreto
dc.contributor.authorNascimento, Francisco X.
dc.date.accessioned2026-04-23T15:49:37Z
dc.date.available2026-04-23T15:49:37Z
dc.date.issued2026-04-01
dc.description.abstractBotrytis cinerea, the causative agent of gray mold, is a major fungal pathogen affecting a wide range of economically important crops. To identify sustainable alternatives to chemical fungicides, this study characterized the biocontrol potential of two bacterial strains, Serratia quinivorans NFX21 and Pseudomonas thivervalensis NFX104, through genomic analysis and functional assays targeting key stages of fungal growth and plant infection. The NFX21 and NFX104 strains significantly inhibited B. cinerea mycelial growth (~35%) and strongly suppressed conidial germination with performances comparable to the reference biocontrol strain Bacillus amyloliquefaciens QST 713. In tomato detached-leaf and whole-plant pot assays, application of NFX21 and NFX104 significantly reduced gray mold incidence and lesion severity relative to nontreated infected plants (53–64%, detached leaves; 12–13%, whole-plant assays), achieving disease control levels similar to those obtained with the commercial biofungicide Serenade ASO®. Whole-genome sequencing allowed the taxonomic assignment of the NFX strains and revealed a rich repertoire of biosynthetic gene clusters and antifungal determinants. The NFX21 genome contained genes associated with N-acyl-homoserine lactone-mediated quorum-sensing and production of lipopeptides, siderophores, and extracellular lytic enzymes. The NFX104 genome harbored clusters involved in the biosynthesis of multiple siderophores, 2,4-diacetylphloroglucinol and hydrogen cyanide. Moreover, both the NFX21 and NFX104 genomes contained additional low-homology clusters that potentially encode for novel unexplored metabolites. Collectively, these results support the translational potential of NFX21 and NFX104 as biocontrol candidates for sustainable, integrated management of gray mold caused by B. cinerea.eng
dc.identifier.doi10.3390/plants15071052
dc.identifier.eid105035629192
dc.identifier.other65a210b5-7231-4b9b-847b-8be5f9215a5b
dc.identifier.pmcPMC13074693
dc.identifier.pmid41977710
dc.identifier.urihttp://hdl.handle.net/10400.14/57576
dc.identifier.wos001739342400001
dc.language.isoeng
dc.peerreviewedyes
dc.publisherMDPI
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.subjectAntifungaleng
dc.subjectBiocontroleng
dc.subjectBotrytis cinereaeng
dc.subjectGray moldeng
dc.subjectPseudomonaseng
dc.subjectSerratiaeng
dc.titleFunctional and genomic characterization of Serratia quinivorans NFX21 and Pseudomonas thivervalensis NFX104, novel biocontrol agents against Botrytis cinerea
dc.typeresearch article
dspace.entity.typePublication
oaire.citation.issue7
oaire.citation.volume15
oaire.versionhttp://purl.org/coar/version/c_970fb48d4fbd8a85

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