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Advisor(s)
Abstract(s)
Continuous cultures of two strains of Clostridium acetobutylicum were stable for over 70 d when grown on glucose/ glycerol mixtures. Butanol was the major fermentation end-product, accounting for 43 to 62% (w/w) of total products. Low-grade glycerol [65% (w/v) purity] could replace commercial glycerol [87% (w/v) purity], leading to
a similar fermentation pattern: a butanol yield of 0.34 (mol/mol), a butanol productivity of 0.42 g l−1 h−1 and a 84%
(w/w) glycerol consumptionwere attained when cultures were grown at pH 6 and D = 0.05 h−1; butanol accounted for 94% (w/w) of total solvents. These values are among the highest reported in literature for C. acetobutylicum simple chemostats.
Description
Keywords
Clostridium acetobutylicum Culture stability Glycerol fermentation Low-grade glycerol
Citation
"Biotechnology letters": ISSN 0141-5492. 25: 2 (2003) 121-125
