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Polyphasic characterization of carbapenemresistant Klebsiella pneumoniae clinical isolates suggests vertical transmission of the blaKPC-3gene

2021-02Journal article Open access
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dc.contributor.authorFerreira, Catarina
dc.contributor.authorBikkarolla, Santosh K.
dc.contributor.authorFrykholm, Karolin
dc.contributor.authorPohjanen, Saga
dc.contributor.authorBrito, Margarida
dc.contributor.authorLameiras, Cataria
dc.contributor.authorNunes, Olga C.
dc.contributor.authorWesterlund, Fredrik
dc.contributor.authorManaia, Célia M.
dc.date.accessioned2021-03-29T18:37:49Z
dc.date.available2021-03-29T18:37:49Z
dc.date.issued2021-02
dc.description.abstractCarbapenem-resistant Klebsiella pneumoniae are a major global threat in healthcare facilities. The propagation of carbapenem resistance determinants can occur through vertical transmission, with genetic elements being transmitted by the host bacterium, or by horizontal transmission, with the same genetic elements being transferred among distinct bacterial hosts. This work aimed to track carbapenem resistance transmission by K. pneumoniae in a healthcare facility. The study involved a polyphasic approach based on conjugation assays, resistance phenotype and genotype analyses, whole genome sequencing, and plasmid characterization by pulsed field gel electrophoresis and optical DNA mapping. Out of 40 K. pneumoniae clinical isolates recovered over two years, five were carbapenem- and multidrug- resistant and belonged to multilocus sequence type ST147. These isolates harboured the carbapenemase encoding blaKPC-3 gene, integrated in conjugative plasmids of 140 kbp or 55 kbp, belonging to replicon types incFIA/incFIIK or incN/incFIIK, respectively. The two distinct plasmids encoding the blaKPC-3 gene were associated with distinct genetic lineages, as confirmed by optical DNA mapping and whole genome sequence analyses. These results suggested vertical (bacterial strain-based) transmission of the carbapenem-resistance genetic elements. Determination of the mode of transmission of antibiotic resistance in healthcare facilities, only possible based on polyphasic approaches as described here, is essential to control resistance propagation.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.doi10.1371/journal.pone.0247058pt_PT
dc.identifier.eid85102074742
dc.identifier.issn1932-6203
dc.identifier.pmcPMC7909683
dc.identifier.pmid33635888
dc.identifier.urihttp://hdl.handle.net/10400.14/32360
dc.identifier.wos000624538400059
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.titlePolyphasic characterization of carbapenemresistant Klebsiella pneumoniae clinical isolates suggests vertical transmission of the blaKPC-3genept_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.issue2pt_PT
oaire.citation.titlePLoS ONEpt_PT
oaire.citation.volume16pt_PT
person.familyNameNunes
person.familyNameWesterlund
person.familyNameManaia
person.givenNameOlga
person.givenNameFredrik
person.givenNameCélia
person.identifier.ciencia-idBA14-BD30-C9AF
person.identifier.ciencia-id951D-5065-FFCB
person.identifier.ciencia-idC31F-553B-6365
person.identifier.orcid0000-0003-4742-2537
person.identifier.orcid0000-0002-4767-4868
person.identifier.orcid0000-0002-3674-1789
person.identifier.ridA-2340-2011
person.identifier.scopus-author-id7005088596
person.identifier.scopus-author-id6505916424
person.identifier.scopus-author-id6602465318
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublication6b4a1abf-b245-41ad-8335-fa0f379650bb
relation.isAuthorOfPublication6105be68-6c20-4be7-8fb4-206a68a669a4
relation.isAuthorOfPublicationa5f14577-0aed-4046-97da-5f4216c7aa07
relation.isAuthorOfPublication.latestForDiscoverya5f14577-0aed-4046-97da-5f4216c7aa07

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