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Differential responses of the antioxidant defence system and ultrastructure in a salt-adapted potato cell line

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dc.contributor.authorQueirós, Filipa
dc.contributor.authorRodrigues, José A.
dc.contributor.authorAlmeida, José M.
dc.contributor.authorAlmeida, Domingos P. F.
dc.contributor.authorFidalgo, Fernanda
dc.date.accessioned2012-01-26T16:54:21Z
dc.date.available2012-01-26T16:54:21Z
dc.date.issued2011
dc.description.abstractChanges in lipid peroxidation and ion content and the possible involvement of the antioxidant system in salt tolerance at the cellular level was studied in a potato (Solanum tuberosum L.) callus line grown on 150 mM NaCl (salt-adapted) and in a non-adapted line exposed to 150 mM NaCl (salt-stressed). Salinity reduced the growth rate and increased lipid peroxidation in salt-stressed line, which remained unaltered in the adapted line. Na+ and Cl− content increased due to salinity in both lines, but the adapted line displayed greater K+/Na+ ratio than the stressed one. Total superoxide dismutase (SOD, EC 1.15.1.1), ascorbate peroxidase (APX, EC 1.11.1.11), and glutathione reductase (GR, EC 1.6.4.2) activities decreased in both salt-exposed lines; catalase (CAT, EC 1.11.1.6) activity did not change in the adapted line, but decreased in the stressed cell line. Salinity caused the suppression of one GR isoform, while the isozyme patterns of SOD, APX, and CAT were not affected. Ascorbate and reduced glutathione increased in both salt-exposed calli lines. α-Tocopherol increased as a result of salt exposure, with higher levels found in adapted calli. Electron microscopy showed that neither the structural integrity of the cells nor membrane structure were affected by salinity, but plastids from adapted cells had higher starch content. The results suggest that the enzymic and non-enzymic components of the antioxidant system are differentially modulated by salt. Different concentrations of antioxidant metabolites are more relevant to the adaptive response to salinity in potato calli than the differences in activity of the antioxidant enzymes.por
dc.description.versioninfo:eu-repo/semantics/acceptedVersion
dc.identifier.citationQUEIRÓS, Filipa... [et al.] - Differential responses of the antioxidant defence system and ultrastructure in a salt-adapted potato cell line. Plant Physiology and Biochemistry. ISSN 0981-9428. Vol. 49, n.º 12 (2011), p. 1410-1419por
dc.identifier.doi10.1016/j.plaphy.2011.09.020
dc.identifier.eid80054106820
dc.identifier.issn0981-9428
dc.identifier.pmid22078378
dc.identifier.urihttp://hdl.handle.net/10400.14/7639
dc.identifier.wosWOS:000297879400005
dc.language.isoengpor
dc.peerreviewedyespor
dc.publisherElsevierpor
dc.subjectAntioxidant defence systempor
dc.subjectCallus tissuepor
dc.subjectElectron microscopypor
dc.subjectOxidative stresspor
dc.subjectSalinitypor
dc.subjectSolanum tuberosumpor
dc.titleDifferential responses of the antioxidant defence system and ultrastructure in a salt-adapted potato cell linepor
dc.typejournal article
dspace.entity.typePublication
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/SFRH/SFRH%2FBD%2F18037%2F2004/PT
oaire.citation.endPage1419
oaire.citation.issue12
oaire.citation.startPage1410
oaire.citation.titlePlant Physiology and Biochemistry
oaire.citation.volume49
oaire.fundingStreamSFRH
person.familyNameQueirós
person.familyNameRodrigues
person.familyNameAlmeida
person.familyNameda Silva Fidalgo Ferro de Beça
person.givenNameAna Filipa
person.givenNameJosé António
person.givenNameDomingos
person.givenNameMaria Fernanda
person.identifier215276
person.identifierhttps://scholar.google.pt/citations?user=6UPnC5cAAAAJ&hl=pt-PT
person.identifier.ciencia-id6713-0CAC-3319
person.identifier.ciencia-id4A1C-3888-D945
person.identifier.ciencia-id4A15-61A1-3532
person.identifier.orcid0000-0002-4161-2973
person.identifier.orcid0000-0002-3950-528X
person.identifier.orcid0000-0001-8429-8585
person.identifier.orcid0000-0001-7756-5243
person.identifier.ridD-4567-2013
person.identifier.ridF-1835-2010
person.identifier.scopus-author-id56427865500
person.identifier.scopus-author-id7202707347
person.identifier.scopus-author-id14827787500
person.identifier.scopus-author-id6602504779
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsrestrictedAccesspor
rcaap.typearticlepor
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