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Cytotoxicity and genotoxicity of chitooligosaccharides upon lymphocytes

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dc.contributor.authorFernandes, João C.
dc.contributor.authorBorges, Margarida
dc.contributor.authorNascimento, Henrique
dc.contributor.authorBronze-da-Rocha, Elsa
dc.contributor.authorRamos, Oscar S.
dc.contributor.authorPintado, Manuela E.
dc.contributor.authorMalcata, F. Xavier
dc.contributor.authorSantos-Silva, Alice
dc.date.accessioned2012-02-27T15:06:44Z
dc.date.available2012-02-27T15:06:44Z
dc.date.issued2011
dc.description.abstractTwo COS mixtures and a low molecular weight chitosan (LMWC) were tested for potential cytotoxicity and genotoxicity upon human lymphocytes. Genotoxicity was evaluated in vitro by cytokinesis-blocked micronucleus and alkaline comet assays, while cytotoxicity was assessed by flow cytometry analysis. Our results suggest that COS do not exhibit any genotoxicity upon human lymphocytes, independently of MW or concentration. However, above 0.07 mg/mL COS induced strong cytotoxic effects. According to the concentration used, such cytotoxicity will induce cell death, essentially by necrosis (>0.10 mg/mL) and/or apoptosis (<0.10 mg/mL). The level of necrosis/apoptosis induced by high COS concentrations, suggests a promising use as apoptosis inducers in specific cancer situations.por
dc.description.versioninfo:eu-repo/semantics/publishedVersion
dc.identifier.citationFERNANDES, João C... [et al.] - Cytotoxicity and genotoxicity of chitooligosaccharides upon lymphocytes. International Journal of Biological Macromolecules. ISSN 0141-8130. Vol. 49, n.º3 (2011), p. 433-438por
dc.identifier.doi10.1016/j.ijbiomac.2011.05.032
dc.identifier.eissn1879-0003
dc.identifier.issn0141-8130
dc.identifier.urihttp://hdl.handle.net/10400.14/7952
dc.identifier.wosWOS:000292853900029
dc.language.isoengpor
dc.peerreviewedyespor
dc.publisherElsevierpor
dc.subjectMicronucleuspor
dc.subjectComet assaypor
dc.subjectFlow cytometrypor
dc.subjectChitooligosaccharidespor
dc.subjectLymphocytespor
dc.titleCytotoxicity and genotoxicity of chitooligosaccharides upon lymphocytespor
dc.typejournal article
dspace.entity.typePublication
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/SFRH/SFRH%2FBD%2F31087%2F2006/PT
oaire.citation.endPage438
oaire.citation.issue3
oaire.citation.startPage433
oaire.citation.titleInternational Journal of Biological Macromolecules
oaire.citation.volume49
oaire.fundingStreamSFRH
person.familyNameFernandes
person.familyNameBorges
person.familyNameNascimento
person.familyNameBronze da Rocha
person.familyNameRamos
person.familyNamePintado
person.familyNameMalcata
person.familyNameSantos-Silva
person.givenNameJoão
person.givenNameMargarida
person.givenNameHenrique
person.givenNameElsa
person.givenNameOscar
person.givenNameMaria Manuela
person.givenNameFrancisco
person.givenNameAlice
person.identifier456608
person.identifier1368242
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person.identifier.scopus-author-id7102542478
person.identifier.scopus-author-id6603836490
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsrestrictedAccesspor
rcaap.typearticlepor
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