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Development and validation method for simultaneous quantification of phenolic compounds in natural extracts and nanosystems

2013Journal article Restricted access
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dc.contributor.authorSilva, Sara Baptista da
dc.contributor.authorOliveira, Ana
dc.contributor.authorFerreira, Domingos
dc.contributor.authorSarmento, Bruno
dc.contributor.authorPintado, Manuela
dc.date.accessioned2014-05-15T12:12:31Z
dc.date.available2014-05-15T12:12:31Z
dc.date.issued2013
dc.description.abstractIntroductionSage and savoury (Salvia sp. and Satureja montana, respectively) are plants used in traditional medicine. The quality control of their herbal formulations is of paramount concern to guarantee the expected biological activity of their anti-oxidant compounds. Objective To establish a simple and effective high-performance liquid chromatographic (HPLC) method to evaluate simultaneously quercetin and rosmarinic acid, in a pure form, in natural extracts (sage and savoury), and encapsulated into chitosan nanoparticles. Methods Chromatography was performed on an RP C-18-column, in a gradient mode with a mobile phase comprising methanol:formic acid:water 92.5:2.5:5 (v/v) at a flow rate of 0.75mL/min and at wavelength of 280nm. Results The method was specific, linear in the range of 0.05-1mg/mL (R-2=1.00), precise at the intraday and interday levels, accurate (recovery rate 90.50.6%), and robust to changes in equipment conditions. Conclusion The method established was effective for quercetin and rosmarinic acid characterisation in natural extracts and in chitosan nanoparticles, allowing the loading capacity determination, the association efficiency as well as the in vitro release. This study shows a new HPLC method validated to simultaneous quantification of rosmarinic acid and quercetin in natural extracts and chitosan nanoparticles. This method is also able for detection and quantification of other phenolic and flavanoid compounds in natural matrix.por
dc.description.versioninfo:eu-repo/semantics/submittedVersion
dc.identifier.citationSILVA, S… [et al.] - Development and Validation Method for Simultaneous Quantification of Phenolic Compounds in Natural Extracts and Nanosystems. Phytochemical Analysis. ISSN 1099-1565. Vol. 24, N.º 6 (2013), p. 638-644por
dc.identifier.doi10.1002/pca.2446
dc.identifier.eissn1099-1565
dc.identifier.issn0958-0344
dc.identifier.urihttp://hdl.handle.net/10400.14/14431
dc.identifier.wosWOS:000326032100017
dc.language.isoengpor
dc.peerreviewedyespor
dc.publisherWileypor
dc.relationStrategic Project - LA 16 - 2011-2012
dc.relationANTIOXIDANT CONTAINING CHITOSAN NANOMEDICINES FOR THE PREVENTION AND TREATMENT OF DIABETIC RETINOPATHY
dc.subjectHPLCpor
dc.subjectChitosan nanoparticlespor
dc.subjectQuercetinpor
dc.subjectRosmarinic acidpor
dc.titleDevelopment and validation method for simultaneous quantification of phenolic compounds in natural extracts and nanosystemspor
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleStrategic Project - LA 16 - 2011-2012
oaire.awardTitleANTIOXIDANT CONTAINING CHITOSAN NANOMEDICINES FOR THE PREVENTION AND TREATMENT OF DIABETIC RETINOPATHY
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/PEst-OE%2FEQB%2FLA0016%2F2011/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT//SFRH%2FBD%2F61423%2F2009/PT
oaire.citation.endPage644
oaire.citation.issue6
oaire.citation.startPage638
oaire.citation.titlePhytochemical Analysis
oaire.citation.volume24
oaire.fundingStream6817 - DCRRNI ID
person.familyNameBaptista da Silva
person.familyNameOliveira
person.familyNameFerreira
person.familyNameSarmento
person.familyNamePintado
person.givenNameSara
person.givenNameAna
person.givenNameDomingos
person.givenNameBruno
person.givenNameMaria Manuela
person.identifier404100
person.identifier1415634
person.identifier152609
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person.identifier.ciencia-idC510-41A8-08DC
person.identifier.ciencia-id4713-31C8-A356
person.identifier.ciencia-id1C18-A963-BC5E
person.identifier.ciencia-idE816-B6B0-06A5
person.identifier.ciencia-id2F13-AAE0-3405
person.identifier.orcid0000-0003-1805-144X
person.identifier.orcid0000-0001-8012-4203
person.identifier.orcid0000-0001-6200-7169
person.identifier.orcid0000-0001-5763-7553
person.identifier.orcid0000-0002-0760-3184
person.identifier.ridJ-3404-2013
person.identifier.ridM-7978-2017
person.identifier.ridJ-6265-2013
person.identifier.ridF-5696-2013
person.identifier.scopus-author-id50060987900
person.identifier.scopus-author-id55937245800
person.identifier.scopus-author-id56871285000
person.identifier.scopus-author-id57194334236
person.identifier.scopus-author-id7004483898
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsrestrictedAccesspor
rcaap.typearticlepor
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