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The impact of antibiotic exposure in water and zebrafish gut microbiomes: a 16S rRNA gene-based metagenomic analysis

2019Journal article Open access
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dc.contributor.authorAlmeida, Ana Rita
dc.contributor.authorAlves, Marta
dc.contributor.authorDomingues, Inês
dc.contributor.authorHenriques, Isabel
dc.date.accessioned2019-10-21T18:21:32Z
dc.date.available2019-10-21T18:21:32Z
dc.date.issued2019
dc.description.abstractIn order to supply human demand for food, the aquaculture industry has been growing fast in the last years, being fish usually cultivated in overcrowded conditions. Hence, to prevent the rapidly disease spreading, antibiotics may be applied to both sick and healthy animals. Due to its broad spectrum, oxytetracycline (OTC) is one of the most used antibiotics in food-production. Yet, although useful to prevent infections, antibiotics may reshape aquatic animals' microbiome, disturbing hosts’ welfare. However, the impact of this exposure to the organism microbiome and its surrounding environment is poorly understood. Then, the objective of this study was to analyze in detail the long-term effect of OTC in both zebrafish gut and water microbiomes. Zebrafish adults were exposed, via water, for two months to three concentrations of OTC (0, 10 and 10000 μg/L). Total DNA was extracted from gut and water samples and the V3–V4 region of the bacterial 16 S rRNA gene was sequenced using Illumina technology. Results of alpha and beta-diversity analyses revealed that long-term exposure to OTC impacted both zebrafish gut and water microbiomes. In water samples, effects were observed even at the lowest (10 μg/L) OTC concentration tested resulting in an increase in Deltaproteobacteria, namely the Myxococcales and Bdellovibrionales orders. On the other hand, effects on zebrafish gut were only observed at the highest concentration with the selection of Alphaproteobacteria and Actinobacteria classes. Although these classes are common in fish gut, the increase of Actinobacteria may represent a health problem since some genera like Gordonia are linked to some human infection disease. Nevertheless, in both gut and water, it was observed a decrease in Gamaproteobacteria, probably due to OTC mode of action. In silico functional metagenomic analysis revealed that OTC exposure selected general detoxification mechanisms. In addition, the abundance of functional genes involved in Quorum Sensing (QS) increased under OTC exposure suggesting that QS may help bacteria to survive OTC stress. Thus, future studies should consider post-exposure scenarios for a deeper analysis of the water and zebrafish gut resistome, since bacteria may react differently after exposure ceased.pt_PT
dc.description.versioninfo:eu-repo/semantics/acceptedVersionpt_PT
dc.identifier.citationAlmeida, A. R., Alves, M., Domingues, I., & Henriques, I. (2019). The impact of antibiotic exposure in water and zebrafish gut microbiomes: a 16S rRNA gene-based metagenomic analysis. Ecotoxicology and Environmental Safety, 186, 109771. https://doi.org/10.1016/j.ecoenv.2019.109771pt_PT
dc.identifier.doi10.1016/j.ecoenv.2019.109771pt_PT
dc.identifier.eid85073241872
dc.identifier.eissn1090-2414
dc.identifier.issn0147-6513
dc.identifier.pmid31629904
dc.identifier.urihttp://hdl.handle.net/10400.14/28464
dc.identifier.wos000496901100019
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherElsevierpt_PT
dc.relationCentre for Environmental and Marine Studies
dc.relationLong term consequences of chemical exposure in zebrafish different life stages and potential role of the associated microbiome in the observed toxicity
dc.subjectTetracyclinept_PT
dc.subjectMicrobiomept_PT
dc.subjectOTUpt_PT
dc.subjectPiphillinpt_PT
dc.subjectDanio reriopt_PT
dc.titleThe impact of antibiotic exposure in water and zebrafish gut microbiomes: a 16S rRNA gene-based metagenomic analysispt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleCentre for Environmental and Marine Studies
oaire.awardTitleLong term consequences of chemical exposure in zebrafish different life stages and potential role of the associated microbiome in the observed toxicity
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UID%2FAMB%2F50017%2F2019/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT//PD%2FBD%2F127811%2F2016/PT
oaire.citation.titleEcotoxicology and Environmental Safetypt_PT
oaire.citation.volume186
oaire.fundingStream6817 - DCRRNI ID
person.familyNameAlves
person.familyNameHenriques
person.givenNameMarta
person.givenNameIsabel
person.identifier.ciencia-idD214-4F83-1445
person.identifier.ciencia-idF516-BFA6-1588
person.identifier.orcid0000-0003-1060-058X
person.identifier.orcid0000-0001-7717-4939
person.identifier.ridO-7380-2017
person.identifier.scopus-author-id55362894100
person.identifier.scopus-author-id6701586581
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublication51db2775-48fe-4e93-b9c9-a1e6f5e1515b
relation.isAuthorOfPublication8ff1967b-6347-42bc-aa4e-ff16d1bf4f32
relation.isAuthorOfPublication.latestForDiscovery8ff1967b-6347-42bc-aa4e-ff16d1bf4f32
relation.isProjectOfPublication58d368ef-1c6a-4986-9479-37f5292e16bf
relation.isProjectOfPublication16106c62-4d64-4b36-8647-29e760b7f631
relation.isProjectOfPublication.latestForDiscovery58d368ef-1c6a-4986-9479-37f5292e16bf

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