| dc.contributor.author | Martingo, Maria | |
| dc.contributor.author | Baptista-Silva, Sara | |
| dc.contributor.author | Mesquita, Raquel | |
| dc.contributor.author | Ferreira, João Paulo | |
| dc.contributor.author | Borges, Sandra | |
| dc.contributor.author | Pintado, Manuela | |
| dc.date.accessioned | 2025-02-24T18:24:14Z | |
| dc.date.available | 2025-02-24T18:24:14Z | |
| dc.date.issued | 2024 | |
| dc.description.abstract | Chronic kidney disease (CKD) is characterized by a continuous decline in kidney function, often persisting for years or even a lifetime. This study presents a new exploration into the extraction and application of insect chitosan (Tenebrio molitor) targeting hemodialysis (HD). Initially, the potential of insect-derived chitosan as a sustainable and effective alternative to traditional sources (i.e. crustaceans) is demonstrated. Efficient extraction methods were developed (totaling between 6 h and 12 h), producing chitosan. Firstly, chitin was efficiently isolated from T. molitor through deproteinization and demineralization, with yields of approximately 5% (w/w). Subsequently, chitosan was obtained from the extracted chitin resulting in yields of between 65.0 ± 0.8% (w/w) and 79.3 ± 0.8% (w/w). Characterization using Fourier Transform Infrared Spectroscopy (FTIR) confirmed structural similarities with commercial chitosan and degrees of deacetylation in the 73 - 75% range. The bioactive properties of chitosan obtained from T. molitor, including antimicrobial and antioxidant activities, were evaluated. All the microorganisms tested were inhibited, exhibiting minimum lethal concentrations between 2 and 8 mg/mL, confirming the antimicrobial activity of the extracted chitosan. In addition, chitosan showed antioxidant activity in the range of 60 to 65 µmol Trolox equivalent/g, suggesting its viability for various medical applications. This study also developed a chitosan-based membrane (CH - M) culminating in a proof-of-concept phase for hemodialysis. The permeation characteristics of CH-M for urea and albumin were studied in vitro to assess their suitability as HD membranes. Urea was permeable to values of over 70% and albumin was retained. | pt_PT |
| dc.description.version | info:eu-repo/semantics/publishedVersion | pt_PT |
| dc.identifier.doi | 10.20944/preprints202404.1094.v1 | pt_PT |
| dc.identifier.uri | http://hdl.handle.net/10400.14/48255 | |
| dc.language.iso | eng | pt_PT |
| dc.peerreviewed | no | pt_PT |
| dc.publisher | Preprints.org | pt_PT |
| dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | pt_PT |
| dc.subject | Tenebrio molitor | pt_PT |
| dc.subject | Chitosan | pt_PT |
| dc.subject | Bio-based membrane | pt_PT |
| dc.subject | Hemodialysis treatment | pt_PT |
| dc.title | An uplifting avenue upon mealworm chitosan for hemodialysis application | pt_PT |
| dc.type | preprint | |
| dspace.entity.type | Publication | |
| oaire.citation.endPage | 16 | pt_PT |
| oaire.citation.startPage | 1 | pt_PT |
| rcaap.rights | openAccess | pt_PT |
| rcaap.type | preprint | pt_PT |
