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Lipid class composition of the microalga Pavlova Lutheri: Eicosapentaenoic and docosahexaenoic acids

dc.contributor.authorMeireles, Luís A.
dc.contributor.authorGuedes, A. Catarina
dc.contributor.authorMalcata, F. Xavier
dc.date.accessioned2011-10-23T09:08:44Z
dc.date.available2011-10-23T09:08:44Z
dc.date.issued2003
dc.description.abstractThe lipid classes of Pavlova lutheri, cultivated in semicontinuous mode, were studied by thin-layer chromatography and gas chromatography in attempts to describe the distribution of fatty acid residues within its lipid pool, with special emphasis on eicosapentaenoic (C20:5n−3, EPA) and docosahexaenoic (C22:6n−3, DHA) acids. Neutral lipids and glycolipids were the major constituents and accounted for 57 and 24% of the total fatty acid residues (TFA), respectively. Phospholipids accounted for 10% of TFA. Two lipid classes, acylated steryl glycosides (SG) and diphosphatidylglycerols (DPG), were eventually identified in P. lutheri for the first time. The nonpolar fraction was mainly composed of triacylglycerol (TAG), whereas the polar fraction was mainly composed of monogalactosylacylglycerols (MGDG). The distribution of total EPA and DHA within the lipid pool was calculated in attempts to ascertain the quality of said microalgae as a feed source, as well as the possibility of enhancement of individual fatty acid production and extraction thereafter. EPA was especially concentrated in MGDG (45%) and TAG (33%); conversely, DHA was dispersed through various classes, especially within TAG (27%), DPG (22%), and betaine lipids (21%).por
dc.identifier.citationMEIRELES, Luís A. ; GUEDES, A. Catarina ; MALCATA, F. Xavier - Lipid Class Composition of the Microalga Pavlova lutheri: Eicosapentaenoic and Docosahexaenoic Acids. Journal of Agricultural and Food Chemistry. ISSN: 1520-5118. Vol. 51, n.º 8 (2003)p. 2237–2241por
dc.identifier.urihttp://hdl.handle.net/10400.14/6915
dc.language.isoengpor
dc.peerreviewedyespor
dc.publisherACS Publicationpor
dc.subjectDHApor
dc.subjectEPApor
dc.subjectLipid classespor
dc.subjectThin-layer chromatographypor
dc.subjectAquaculturepor
dc.titleLipid class composition of the microalga Pavlova Lutheri: Eicosapentaenoic and docosahexaenoic acidspor
dc.typejournal article
dspace.entity.typePublication
person.familyNameMalcata
person.givenNameFrancisco
person.identifier.ciencia-id1B13-38A5-35F5
person.identifier.orcid0000-0003-3073-1659
person.identifier.scopus-author-id7102542478
rcaap.rightsrestrictedAccesspor
rcaap.typearticlepor
relation.isAuthorOfPublicationa06c00da-0e2f-4434-8ede-4d7b22c0dfe9
relation.isAuthorOfPublication.latestForDiscoverya06c00da-0e2f-4434-8ede-4d7b22c0dfe9

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