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Biofunctional silk sericin hydrogels: a versatile platform with potential for tissue healing and regeneration

2025-11-01Artigo de investigação Acesso aberto
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dc.contributor.authorVeiga, Anabela
dc.contributor.authorRibeiro, Viviana
dc.contributor.authorRamírez-Jiménez, Rosa Ana
dc.contributor.authorAguilar, Maria Rosa
dc.contributor.authorRojo, Luis
dc.contributor.authorOliveira, Ana L.
dc.date.accessioned2025-09-02T14:17:46Z
dc.date.available2025-09-02T14:17:46Z
dc.date.issued2025-11-01
dc.description.abstractDiscarded silk sericin protein (SS) presents a high yet underexplored potential as a biomaterial for tissue engineering (TE). Despite its biocompatibility, antioxidant activity, and moisture retention properties, its poor stability in aqueous media has limited broader application. In this work, we developed and characterized SS-based hydrogels using tannic acid (TA) and horseradish peroxidase (HRP) crosslinking systems to address these limitations and expand their use in skin TE. Hydrogels were prepared using SS concentrations of 2.5 % and 5 % (w/v) and evaluated for rheological behavior (G′ ranging from 100 to 10,000 Pa), swelling (up to 24 %), retention capacity (stable over 24–30 h), and degradation in proteolytic environments (mass loss ranging from ∼0–11 %, depending on formulation). TA-crosslinked hydrogels showed strong fluid retention and are suitable for high-moisture 3D wound dressings and coating applications. HRP-crosslinked hydrogels demonstrated tunable mechanical properties, shear-thinning behavior, and full recovery post-deformation, making them ideal for use as bioinks in 3D bioprinting and injectable matrices. In vitro assays confirmed cytocompatibility, with viability exceeding 85 %, and successful cell encapsulation and proliferation. Overall, this study presents a versatile SS-based hydrogel platform with potential for various biomedical applications, particularly in skin tissue healing and regeneration.eng
dc.identifier.citationVeiga, A., Ribeiro, V., Ramírez-Jiménez, R. A., & Aguilar, M. R. et al. (2025). Biofunctional silk sericin hydrogels: a versatile platform with potential for tissue healing and regeneration. Colloids and Surfaces B: Biointerfaces, 255, Article 114916. https://doi.org/10.1016/j.colsurfb.2025.114916
dc.identifier.doi10.1016/j.colsurfb.2025.114916
dc.identifier.eid105010466786
dc.identifier.issn0927-7765
dc.identifier.other12334794-f87b-4ac5-a269-334c29fa8e88
dc.identifier.pmid40652876
dc.identifier.urihttp://hdl.handle.net/10400.14/54616
dc.identifier.wos001539419400001
dc.language.isoeng
dc.peerreviewedyes
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/
dc.subjectHorseradish peroxidase (HRP)
dc.subjectHydrogels
dc.subjectSilk sericin (SS)
dc.subjectSkin tissue engineering (TE)
dc.subjectTannic acid (TA)
dc.titleBiofunctional silk sericin hydrogels: a versatile platform with potential for tissue healing and regenerationeng
dc.typeresearch article
dspace.entity.typePublication
oaire.citation.titleColloids and Surfaces B: Biointerfaces
oaire.citation.volume255
oaire.versionhttp://purl.org/coar/version/c_970fb48d4fbd8a85

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