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Advisor(s)
Abstract(s)
Fluorinated compounds are known to be more resistant to microbial degradation than other halogenated
chemicals. A microbial consortium capable of aerobic biodegradation of fluorobenzene (FB) as the sole source
of carbon and energy was isolated by selective enrichment from sediments collected in a drain near an
industrial site. A combination of three microbial strains recovered from the enriched consortium was shown
to be necessary for complete FB mineralization. Two of the strains (F1 and F3) were classified by 16S rRNA
analysis as belonging to the Sphingobacterium/Flavobacterium group, while the third (F4) falls in the -Proteobacteria
group, clustering with Alcaligenes species. Strain F4 was consistently found in the liquid cultures in
a much greater proportion than strains F1 and F3 (86:8:6 for F4, F1, and F3, respectively). Stoichiometric
release of fluoride ions was measured in batch and fed-batch cultures. In batch cultures, the consortium was
able to use FB up to concentrations of 400 mg liter 1 and was able to utilize a range of other organic
compounds, including 4-fluorophenol and 4-fluorobenzoate. To our knowledge this is the first time biodegradation
of FB as a sole carbon source has been reported.
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Keywords
Citation
CARVALHO, M.F....[et al] - a Bacterial consortium able to mineralize fluorobenzene. Applied and Environmental Microbiology.ISSN 0099-2240. Vol. 68 n.º 1 (2002), p. 102-105
Publisher
American Society for Microbiology