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Enantioselective quantification of fluoxetine and norfluoxetine by HPLC in wastewater effluents

2014Journal article Restricted access
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dc.contributor.authorRibeiro, Ana R.
dc.contributor.authorMaia, Alexandra S.
dc.contributor.authorMoreira, Irina S.
dc.contributor.authorAfonso, Carlos M.
dc.contributor.authorCastro, Paula M.L.
dc.contributor.authorTiritan, Maria E.
dc.date.accessioned2014-05-08T14:34:26Z
dc.date.available2014-05-08T14:34:26Z
dc.date.issued2014
dc.description.abstractMicrobial degradation is the most important process to remove organic pollutants in Waste Water Treatment Plants. Regarding chiral compounds this process is normally enantioselective and needs the suitable analytical methodology to follow the removal of both enantiomers in an accurate way. Thus, this paper describes the development and validation of an enantioselective High Performance Liquid Chromatography with Fluorescence Detection (HPLC-FD) method for simultaneous analysis of fluoxetine (FLX) and norfluoxetine (NFLX) in wastewater effluents. Briefly, this method preconcentrated a small volume of wastewater samples (50 mL) on 500 mg Oasis MCX cartridges and used HPLC-FD with a vancomycin-based chiral stationary phase under reversed mode for analyses. The optimized mobile phase was EtOH/aqueous ammonium acetate buffer (92.5/7.5, v/v) at pH 6.8. The effect of EtOH percentage, buffer concentration, pH, column oven temperature and flow rate on chromatographic parameters was systematically investigated. The developed method was validated within the wastewater effluent used in microcosms laboratory assays. Linearity (R2 > 0.99), selectivity and sensitivity were achieved in the range of 4.0–60 ng mL 1 for enantiomers of FLX and 2.0–30 ng mL 1 for enantiomers of NFLX. The limits of detection were between 0.8 and 2.0 ng mL 1 and the limits of quantification were between 2.0 and 4.0 ng mL 1 for both enantiomers of FLX and the enantiomers of its demethylated metabolite NFLX. The validated method was successfully applied and proved to be robust to follow the degradation of both enantiomers of FLX in wastewater samples, during 46 days.por
dc.description.versioninfo:eu-repo/semantics/publishedVersion
dc.identifier.citationRIBEIRO, Ana R… [et al.] - Enantioselective quantification of fluoxetine and norfluoxetine by HPLC in wastewater effluents. Chemosphere. ISSN 0045-6535. Vol. 95 (2014) p. 589–596por
dc.identifier.doi10.1016/j.chemosphere.2013.09.118
dc.identifier.issn0045-6535
dc.identifier.urihttp://hdl.handle.net/10400.14/14329
dc.identifier.wosWOS:000328868400080
dc.language.isoengpor
dc.peerreviewedyespor
dc.publisherElsevierpor
dc.relationCEQUIMED-PEstOE/SAU/UI4040/2011
dc.relationFLUOROPHARMA - Biodegradation and removal of chiral and achiral fluorinated pharmaceuticals from wastewaters
dc.relationStrategic Project - LA 16 - 2011-2012
dc.subjectEnantioseparationpor
dc.subjectFluoxetinepor
dc.subjectNorfluoxetinepor
dc.subjectMacrocyclic antibiotic CSPpor
dc.subjectChirobiotic Vpor
dc.titleEnantioselective quantification of fluoxetine and norfluoxetine by HPLC in wastewater effluentspor
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleFLUOROPHARMA - Biodegradation and removal of chiral and achiral fluorinated pharmaceuticals from wastewaters
oaire.awardTitleStrategic Project - LA 16 - 2011-2012
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/SFRH/SFRH%2FBD%2F64999%2F2009/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/SFRH/SFRH%2FBD%2F86939%2F2012/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/SFRH/SFRH%2FBPD%2F87251%2F2012/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/3599-PPCDT/PTDC%2FEBB-EBI%2F111699%2F2009/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/PEst-OE%2FEQB%2FLA0016%2F2011/PT
oaire.citation.endPage596
oaire.citation.startPage589
oaire.citation.titleChemosphere
oaire.citation.volume95
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oaire.fundingStream3599-PPCDT
oaire.fundingStream6817 - DCRRNI ID
person.familyNameRibeiro
person.familyNameMaia
person.familyNameMoreira
person.familyNameAfonso
person.familyNameCastro
person.familyNameTiritan
person.givenNameAna Rita Lado
person.givenNameAlexandra S.
person.givenNameIrina Susana Sousa
person.givenNameCarlos
person.givenNamePaula
person.givenNameMaria Elizabeth
person.identifier903786
person.identifierK-5841-2014
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person.identifier.ciencia-idD915-BFC0-FB3A
person.identifier.orcid0000-0002-4445-5379
person.identifier.orcid0000-0002-4167-383X
person.identifier.orcid0000-0001-6516-0994
person.identifier.orcid0000-0002-5731-5789
person.identifier.orcid0000-0001-8841-6606
person.identifier.orcid0000-0003-3320-730X
person.identifier.ridK-5841-2014
person.identifier.ridI-5989-2015
person.identifier.ridM-8241-2013
person.identifier.ridK-9492-2014
person.identifier.scopus-author-id57207918943
person.identifier.scopus-author-id55332349300
person.identifier.scopus-author-id36798094100
person.identifier.scopus-author-id36145043600
person.identifier.scopus-author-id7102781782
person.identifier.scopus-author-id6602890981
project.funder.identifierhttp://doi.org/10.13039/501100001871
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project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsrestrictedAccesspor
rcaap.typearticlepor
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