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A rationale for the high limits of quantification of antibiotic resistance genes in soil

2018Journal article Open access
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dc.contributor.authorFortunato, Gianuario
dc.contributor.authorVaz-Moreira, Ivone
dc.contributor.authorBecerra-Castro, Cristina
dc.contributor.authorNunes, Olga C.
dc.contributor.authorManaia, Célia M.
dc.date.accessioned2019-10-21T13:47:12Z
dc.date.available2019-10-21T13:47:12Z
dc.date.issued2018
dc.description.abstractThe determination of values of abundance of antibiotic resistance genes (ARGs) per mass of soil is extremely useful to assess the potential impacts of relevant sources of antibiotic resistance, such as irrigation with treated wastewater or manure application. Culture-independent methods and, in particular, quantitative PCR (qPCR), have been regarded as suitable approaches for such a purpose. However, it is arguable if these methods are sensitive enough to measure ARGs abundance at levels that may represent a risk for environmental and human health. This study aimed at demonstrating the range of values of ARGs quantification that can be expected based on currently used procedures of DNA extraction and qPCR analyses. The demonstration was based on the use of soil samples spiked with known amounts of wastewater antibiotic resistant bacteria (ARB) (Enterococcus faecalis, Escherichia coli, Acinetobacter johnsonii, or Pseudomonas aeruginosa), harbouring known ARGs, and also on the calculation of expected values determined based on qPCR. The limits of quantification (LOQ) of the ARGs (vanA, qnrS, blaTEM, blaOXA, blaIMP, blaVIM) were observed to be approximately 4 log-units per gram of soil dry weight, irrespective of the type of soil tested. These values were close to the theoretical LOQ values calculated based on currently used DNA extraction methods and qPCR procedures. The observed LOQ values can be considered extremely high to perform an accurate assessment of the impacts of ARGs discharges in soils. A key message is that ARGs accumulation will be noticeable only at very high doses. The assessment of the impacts of ARGs discharges in soils, of associated risks of propagation and potential transmission to humans, must take into consideration this type of evidence, and avoid the simplistic assumption that no detection corresponds to risk absence.pt_PT
dc.description.versioninfo:eu-repo/semantics/acceptedVersionpt_PT
dc.identifier.citationFortunato, G., Vaz-Moreira, I., Becerra-Castro, C., Nunes, O. C., & Manaia, C. M. (2018). A rationale for the high limits of quantification of antibiotic resistance genes in soil. Environmental Pollution, 243, 1696–1703. https://doi.org/10.1016/j.envpol.2018.09.128pt_PT
dc.identifier.doi10.1016/j.envpol.2018.09.128pt_PT
dc.identifier.eid85054416046
dc.identifier.eissn1873-6424
dc.identifier.issn0269-7491
dc.identifier.pmid30300875
dc.identifier.urihttp://hdl.handle.net/10400.14/28442
dc.identifier.wos000449892700096
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherElsevierpt_PT
dc.relationPOCI-01-0145-FEDER-006939
dc.relationANtibioticS and mobile resistance elements in WastEwater Reuse applications: risks and innovative solutions
dc.relationNORTE-01-0145-FEDER-000005
dc.relationRELATIONSHIP BETWEEN METAL AND ANTIBIOTIC RESISTANCE: IMPLICATIONS IN AGRICULTURAL CROPS
dc.subjectWastewaterpt_PT
dc.subjectRisk assessmentpt_PT
dc.subjectLOQ - Limit of quantificationpt_PT
dc.subjectLOD - Limit of detectionpt_PT
dc.subjectQuantitative PCRpt_PT
dc.titleA rationale for the high limits of quantification of antibiotic resistance genes in soilpt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleANtibioticS and mobile resistance elements in WastEwater Reuse applications: risks and innovative solutions
oaire.awardTitleRELATIONSHIP BETWEEN METAL AND ANTIBIOTIC RESISTANCE: IMPLICATIONS IN AGRICULTURAL CROPS
oaire.awardURIinfo:eu-repo/grantAgreement/EC/H2020/675530/EU
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/5876/UID%2FMulti%2F50016%2F2013/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/3599-PPCDT/WaterJPI%2F0001%2F2013/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/5876/UID%2FEQU%2F00511%2F2013/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/SFRH/SFRH%2FBPD%2F87360%2F2012/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT//SFRH%2FBPD%2F87152%2F2012/PT
oaire.citation.endPage1703
oaire.citation.startPage1696
oaire.citation.titleEnvironmental Pollutionpt_PT
oaire.citation.volume243
oaire.fundingStreamH2020
oaire.fundingStream5876
oaire.fundingStream3599-PPCDT
oaire.fundingStream5876
oaire.fundingStreamSFRH
person.familyNameFortunato
person.familyNameVaz-Moreira
person.familyNameNunes
person.familyNameManaia
person.givenNameGianuario
person.givenNameIvone
person.givenNameOlga
person.givenNameCélia
person.identifier602434
person.identifier.ciencia-id5312-3898-879E
person.identifier.ciencia-id9E17-544E-800D
person.identifier.ciencia-idBA14-BD30-C9AF
person.identifier.ciencia-idC31F-553B-6365
person.identifier.orcid0000-0003-4232-8250
person.identifier.orcid0000-0001-8912-9836
person.identifier.orcid0000-0003-4742-2537
person.identifier.orcid0000-0002-3674-1789
person.identifier.scopus-author-id16044425800
person.identifier.scopus-author-id7005088596
person.identifier.scopus-author-id6602465318
project.funder.identifierhttp://doi.org/10.13039/501100008530
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameEuropean Commission
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
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