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Stability of a commercial lipase from Mucor javanicus: kinetic modeling of pH and temperature dependencies

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dc.contributor.authorBalcão, Victor M.
dc.contributor.authorOliveira, Teresa A.
dc.contributor.authorMalcata, F. Xavier
dc.date.accessioned2011-10-11T12:06:37Z
dc.date.available2011-10-11T12:06:37Z
dc.date.issued1998
dc.description.abstractThe present communication reports experimental and modelling work pertaining to the independent roles of pH and temperature on deactivation of a crude lipase from Mucor javanicus. Experimental data oflipolytic activities were generated by a classic pH-stat assay on a triolein emulsion following incubation at several pH values for a fixed time, or at several temperatures for various times; postulated models were then fitted by nonlinear fitting to such data. The pH-dependence data were best fit by assumption of three forms of enzyme with increasing states of protonation, with pKa values of 6.2 and 11.3, respectively, where only the intermediate form is stable within the time frame considered. The thermal-dependence data were best fit by assumption of parallel steps of deactivation and rearrangement, with activation energies of 228.8 and 221.7 kJ mol~l, respectively.por
dc.identifier.citationBALCÃO, Victor M; OLIVEIRA, Teresa A.; MALCATA, F. Xavier -Stability of a commercial lipase from Mucor javanicus: kinetic modeling of pH and temperature dependencies. Biocatalysis and Biotransformation. ISSN 1024-2422. Vol. 16 (1998), p. 45-66por
dc.identifier.urihttp://hdl.handle.net/10400.14/5958
dc.language.isoengpor
dc.peerreviewedyespor
dc.publisherInforma Healthcarepor
dc.relation.publisherversionhttp://informahealthcare.com/doi/abs/10.3109/10242429809040110?journalCode=babpor
dc.subjectEnzymepor
dc.subjectPHpor
dc.subjectLipolytic activitypor
dc.subjectMathematical modellingpor
dc.subjectBatch reactorpor
dc.subjectActivationpor
dc.titleStability of a commercial lipase from Mucor javanicus: kinetic modeling of pH and temperature dependenciespor
dc.typejournal article
dspace.entity.typePublication
person.familyNameBalcão
person.familyNameMalcata
person.givenNameVictor
person.givenNameFrancisco
person.identifier.ciencia-idC314-A18C-931B
person.identifier.ciencia-id1B13-38A5-35F5
person.identifier.orcid0000-0003-0772-2834
person.identifier.orcid0000-0003-3073-1659
person.identifier.ridI-2182-2012
person.identifier.scopus-author-id6603449856
person.identifier.scopus-author-id7102542478
rcaap.rightsopenAccesspor
rcaap.typearticlepor
relation.isAuthorOfPublication9b0860f7-99b1-4ded-a483-6435882272d0
relation.isAuthorOfPublicationa06c00da-0e2f-4434-8ede-4d7b22c0dfe9
relation.isAuthorOfPublication.latestForDiscoverya06c00da-0e2f-4434-8ede-4d7b22c0dfe9

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