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Fate of nitrogen during metabolism of whey lactose by Rahnella aquatilis

1999Journal article Restricted access
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dc.contributor.authorPintado, Manuela E.
dc.contributor.authorPintado, Ana I. E.
dc.contributor.authorMalcata, F. Xavier
dc.date.accessioned2011-10-20T14:53:40Z
dc.date.available2011-10-20T14:53:40Z
dc.date.issued1999
dc.description.abstractThe rate of intake of peptides and free amino acids by Rahnella aquatilis was studied, as well as the evolution of concentrations of exopolysaccharide, biomass, and several metabolites. For this purpose, eight wheybased fermentation media were tested: hydrolyzed bovine whey under 1) aerobic and 2) anaerobic conditions; hydrolyzed bovine whey with 3) 2.0% (wt/vol) NaCl or 4) 0.5% (wt/vol) NaCl; 5) plain bovine whey; 6) bovine whey permeate; 7) hydrolyzed caprine whey; and 8) hydrolyzed ovine whey. The lower peptide fraction (<2000 Da) in plain whey, whey permeate, and hydrolyzed whey fermented aerobically was virtually depleted by 24 h. In all experiments, most consumption of free amino acids occurred at initial stages of fermentation. In caprine whey, ovine whey, and bovine whey fermented anaerobically, free Val was present at high levels until the end of fermentation, and the highest production of acetic acid was also observed. In ovine whey, high levels of peptides and free amino acids were recorded, as well as the highest production of several organic acids (except lactic acid) and the lowest viscosity and polysaccharide concentration. The low concentration of peptides and free amino acids in plain whey and whey permeate did not apparently permit active consumption of lactose, whereas lack of molecular oxygen and presence of salt played a strong role toward inhibition of exopolysaccharide production. The qualitative and quantitative profile of the nitrogen fraction likely might have determined the metabolism of lactose and, consequently, conditioned the production of exopolysaccharide and organic acids even though growth of R. aquatilis was not affected similarly.por
dc.identifier.citationPINTADO, Manuela E. ; PINTADO, Ana I.E. ; MALCATA, F. Xavier - Fate of nitrogen during metabolism of whey lactose by Rahnella aquatilis. Journal of Dairy Science. ISSN 0022-0302. Vol. 82, n.º 11 (1999), p. 2315-2326por
dc.identifier.urihttp://hdl.handle.net/10400.14/6516
dc.language.isoengpor
dc.peerreviewedyespor
dc.publisherElsevierpor
dc.subjectPeptidespor
dc.subjectAmino acidspor
dc.subjectExopolysaccharidepor
dc.subjectMicroorganismpor
dc.titleFate of nitrogen during metabolism of whey lactose by Rahnella aquatilispor
dc.typejournal article
dspace.entity.typePublication
person.familyNamePintado
person.familyNamePintado
person.familyNameMalcata
person.givenNameMaria Manuela
person.givenNameAna
person.givenNameFrancisco
person.identifier456608
person.identifier.ciencia-id2F13-AAE0-3405
person.identifier.ciencia-id871B-4017-FC07
person.identifier.ciencia-id1B13-38A5-35F5
person.identifier.orcid0000-0002-0760-3184
person.identifier.orcid0000-0002-4931-708X
person.identifier.orcid0000-0003-3073-1659
person.identifier.ridF-5696-2013
person.identifier.scopus-author-id7004483898
person.identifier.scopus-author-id6602847645
person.identifier.scopus-author-id7102542478
rcaap.rightsrestrictedAccesspor
rcaap.typearticlepor
relation.isAuthorOfPublicationba387c7d-27c9-4016-895c-b35597e91ebc
relation.isAuthorOfPublicationcf43b696-b273-4051-bb79-3a9f8fb63940
relation.isAuthorOfPublicationa06c00da-0e2f-4434-8ede-4d7b22c0dfe9
relation.isAuthorOfPublication.latestForDiscoverycf43b696-b273-4051-bb79-3a9f8fb63940

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