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Advisor(s)
Abstract(s)
Microbial cultures able to degrade xenobiotic compounds are the key element for biological treatment of waste
effluents and are obtained from enrichment processes. In this study, two common enrichment methods, suspension
batch and immobilized continuous, were compared. The main selection factor was the presence of 1,3-dichloro-
2-propanol (1,3-DCP) as the single carbon source. Both methods have successfully enriched microbial consortia
able to degrade 1,3-DCP. When tested in batch culture, the degradation rates of 1,3-DCP by the two consortia
were different, with the consortia obtained by batch enrichment presenting slightly higher rates. A preliminary
morphological and biochemical analysis of the predominant colonial types present in each degrading consortia
revealed the presence of different constituting strains. Three bacterial isolates capable of degrading 1,3-DCP as
single strains were obtained from the batch enrichments. These strains were classified by 16S rRNA analysis as
belonging to the Rhizobiaceae group. Degradation rates of 1,3-DCP were lower when single species were used,
reaching 45 mg l−1 d−1, as compared to 74 mg l−1 d−1 of the consortia enriched on the batch method. Mutualistic
interactions may explain the better performance of the enriched consortia.
Description
Keywords
Batch method Continuous method Microbial enrichment
Citation
BASTOS, Filipe...[et al] - Enrichment of microbial cultures able to degrade 1,3-dichloro-2 propanol: A comparison between batch and continuous methods. Biodegradation. ISSN 0923-9820. Vol. 13:(2002), p. 211–220
Publisher
Kluwer Academic Publishers