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Continuous ozonation of urban wastewater: removal of antibiotics, antibiotic-resistant Escherichia coli and antibiotic resistance genes and phytotoxicity

2019Journal article Open access
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dc.contributor.authorIakovides, I. C.
dc.contributor.authorMichael-Kordatou, I.
dc.contributor.authorMoreira, N. F. F.
dc.contributor.authorRibeiro, A. R.
dc.contributor.authorFernandes, T.
dc.contributor.authorPereira, M. F. R.
dc.contributor.authorNunes, O. C.
dc.contributor.authorManaia, C. M.
dc.contributor.authorSilva, A. M. T.
dc.contributor.authorFatta-Kassinos, D.
dc.date.accessioned2019-06-07T11:50:38Z
dc.date.available2019-06-07T11:50:38Z
dc.date.issued2019
dc.description.abstractThis work evaluated the removal of a mixture of eight antibiotics (i.e. ampicillin (AMP), azithromycin (AZM), erythromycin (ERY), clarithromycin (CIA), ofloxacin (OFL), sulfamethoxazole (SMX), trimethoprim (TMP) and tetracycline (TC)) from urban wastewater, by ozonation operated in continuous mode at different hydraulic retention times (HRTs) (i.e. 10, 20, 40 and 60 min) and specific ozone doses (i.e. 0.125, 0.25, 0.50 and 0.75 gO(3) gDOC(-1)). As expected, the efficiency of ozonation was highly ozone dose-and contact time-dependent. The removal of the parent compounds of the selected antibiotics to levels below their detection limits was achieved with HRT of 40 min and specific ozone dose of 0.125 gO(3) gDOC(-1). The effect of ozonation was also investigated at a microbiological and genomic level, by studying the efficiency of the process with respect to the inactivation of Escherichia coli and antibiotic-resistant E. coli, as well as to the reduction of the abundance of selected antibiotic resistance genes (ARG5). The inactivation of total cultivable E. coli was achieved under the experimental conditions of HRT 40 min and 0.25 gO(3) gDOC(-1), at which all antibiotic compounds were already degraded. The regrowth examinations revealed that higher ozone concentrations were required for the permanent inactivation of E. coli below the Limit of Quantification (<LOQ = 0.01 CFU mL(-1)). Also, the abundance of the examined ARGs (intl1, addA1, dfrA1, qacE Delta 1 and sal1) was found to decrease with increasing HRT and ozone dose. Despite the fact that the mildest operating parameters were able to eliminate the parent compounds of the tested antibiotics in wastewater effluents, it was clearly demonstrated in this study that higher ozone doses were required in order to confer permanent damage and/or death and prevent potential post-treatment re-growth of both total bacteria and ARB, and to reduce the abundance of ARGs below the LOQ, Interestingly, the mineralization of wastewater, in terms of Dissolved Organic Carbon (DOC) removal, was found to be significantly low even when the higher ozone doses were applied, leading to an increased phytotoxicity towards various plant species. The findings of this study clearly underline the importance of properly optimising the ozonation process (e.g. specific ozone dose and contact time) taking into consideration both the bacterial species and associated ARGs, as well as the wastewater physicochemical properties (e.g. DOC), in order to mitigate the spread of ARB&ARGs, as well as to reduce the potential phytotoxicity.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationIakovides, I. C., Michael-Kordatou, I., Moreira, N. F. F., Ribeiro, A. R., Fernandes, T., Pereira, M. F. R., … Fatta-Kassinos, D. (2019). Continuous ozonation of urban wastewater: removal of antibiotics, antibiotic-resistant Escherichia coli and antibiotic resistance genes and phytotoxicity. Water Research, 159, 333–347. https://doi.org/10.1016/j.watres.2019.05.025pt_PT
dc.identifier.doi10.1016/j.watres.2019.05.025pt_PT
dc.identifier.eid85065732510
dc.identifier.issn0043-1354
dc.identifier.pmid31108362
dc.identifier.urihttp://hdl.handle.net/10400.14/27728
dc.identifier.wos000472126200035
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherElsevierpt_PT
dc.relationPOCI-01-0145-FEDER-006939
dc.relationANtibioticS and mobile resistance elements in WastEwater Reuse applications: risks and innovative solutions
dc.relationDevelopment of a miniaturized sample preparation prototype for identification and quantification of trace pharmaceuticals in tap waters
dc.relationAdvanced wastewater treatment: organic micropollutants, human pathogens and antibiotic resistance genes
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/pt_PT
dc.subjectAntibioticspt_PT
dc.subjectAntibiotic resistancept_PT
dc.subjectPhytotoxicitypt_PT
dc.subjectOzonationpt_PT
dc.subjectContinuous modept_PT
dc.titleContinuous ozonation of urban wastewater: removal of antibiotics, antibiotic-resistant Escherichia coli and antibiotic resistance genes and phytotoxicitypt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleANtibioticS and mobile resistance elements in WastEwater Reuse applications: risks and innovative solutions
oaire.awardTitleDevelopment of a miniaturized sample preparation prototype for identification and quantification of trace pharmaceuticals in tap waters
oaire.awardTitleAdvanced wastewater treatment: organic micropollutants, human pathogens and antibiotic resistance genes
oaire.awardURIinfo:eu-repo/grantAgreement/EC/H2020/675530/EU
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/FARH/SFRH%2FBPD%2F101703%2F2014/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT//PD%2FBD%2F114318%2F2016/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/5876/UID%2FMulti%2F50016%2F2013/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/5876/UID%2FEQU%2F00511%2F2013/PT
oaire.citation.endPage347
oaire.citation.startPage333
oaire.citation.titleWater Researchpt_PT
oaire.citation.volume159
oaire.fundingStreamH2020
oaire.fundingStreamFARH
oaire.fundingStream5876
oaire.fundingStream5876
person.familyNameMoreira
person.familyNameRibeiro
person.familyNamePereira
person.familyNameNunes
person.familyNameManaia
person.familyNameSilva
person.familyNameFatta-Kassinos
person.givenNameNuno
person.givenNameAna Rita Lado
person.givenNameManuel Fernando
person.givenNameOlga
person.givenNameCélia
person.givenNameAdrián
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project.funder.identifierhttp://doi.org/10.13039/501100008530
project.funder.identifierhttp://doi.org/10.13039/501100001871
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project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameEuropean Commission
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
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