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Can growth of nannochloropsis oculata under modulated stress enhance its lipid-associated biological properties?

2022-11-24Journal article Open access
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dc.contributor.authorSousa, Sérgio C.
dc.contributor.authorMachado, Manuela
dc.contributor.authorFreitas, Ana C.
dc.contributor.authorGomes, Ana M.
dc.contributor.authorCarvalho, Ana P.
dc.date.accessioned2023-01-11T17:17:20Z
dc.date.available2023-01-11T17:17:20Z
dc.date.issued2022-11-24
dc.description.abstractNannochloropsis oculata is well-recognized as a potential microalgal source of valuable compounds such as polyunsaturated fatty acids, particularly, eicosapentaenoic acid (EPA). The content and profile of these lipids is highly dependent on the growth conditions and can, therefore, be tailored through modulation of the growth parameters, specifically, temperature. Moreover, biological activities are composition dependent. In the present work, lipid extracts obtained from N. oculata, grown under constant temperature and under modulated temperature stress (to increase EPA content; Str) were characterized by GC-FID and several bioactivities were evaluated, namely, antioxidant (L-ORACFL), cytotoxic (MTT), adipolytic, anti-hepatic lipid accumulation (steatosis), and anti-inflammatory properties. Both extracts exhibited antioxidant activity (c.a. 49 µmol Troloxequivalent/mgextract) and the absence of toxicity (up to 800 µg/mL) toward colon and hepatic cells, adipocytes, and macrophages. They also induced adipolysis and the inhibition of triglycerides hepatic accumulation, with a higher impact from Str. In addition, anti-inflammatory activity was observed in the lipopolysaccharide-induced inflammation of macrophages in the presence of either extract, since lower levels of pro-inflammatory interleukin-6 and interferon-β were obtained, specifically by Str. The results presented herein revealed that modulated temperature stress may enhance the health effects of N. oculata lipid extracts, which may be safely utilized to formulate novel food products.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.doi10.3390/md20120737pt_PT
dc.identifier.eid85144483723
dc.identifier.issn1660-3397
dc.identifier.pmcPMC9782458
dc.identifier.pmid36547884
dc.identifier.urihttp://hdl.handle.net/10400.14/39822
dc.identifier.wos000902554300001
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectAdipolysispt_PT
dc.subjectAnti-inflammatory activitypt_PT
dc.subjectAntioxidant capacitypt_PT
dc.subjectCytokinespt_PT
dc.subjectEicosapentaenoic acidpt_PT
dc.subjectMetabolic activitypt_PT
dc.subjectPolyunsaturated fatty acidspt_PT
dc.subjectSteatosispt_PT
dc.titleCan growth of nannochloropsis oculata under modulated stress enhance its lipid-associated biological properties?pt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.issue12pt_PT
oaire.citation.titleMarine Drugspt_PT
oaire.citation.volume20pt_PT
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT

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