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Hydrolysis of caseins by extracts of Cynara Cardunculus precipitated by ammonium sulfate

2002Journal article Restricted access
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dc.contributor.authorSilva, S. V.
dc.contributor.authorBarros, R. M.
dc.contributor.authorMalcata, F. Xavier
dc.date.accessioned2011-10-22T12:04:33Z
dc.date.available2011-10-22T12:04:33Z
dc.date.issued2002
dc.description.abstractPolyacrylamide gel electrophoresis (in the presence of urea) and gel permeation chromatography were employed to assess the profile of hydrolysis of caseins and the activity of enzymes contributed by the flowers of the plant Cynara cardunculus on bovine caseins, after previous precipitation with ammonium sulfate or in a plain crude aqueous extract. Results indicated that the qualitative degradation profile of bovine caseins by plant enzymes (cardosins) remains essentially unchanged upon extraction, and quantitative analysis showed that the precipitated fractions had a higher coagulant-to-proteolytic activity ratio; hence, the results showed that inexpensive precipitation with ammonium sulfate can successfully be used as a purification method in the production of that plant coagulant in standardized form.por
dc.identifier.citationSILVA, S.V.; BARROS, R.M.; MALCATA, F.X. - Hydrolysis of caseins by extracts of Cynara Cardunculus precipitated by ammonium sulfate. Journal of Food Science. ISSN 0022-1147. Vol. 67, n.º 5 (2002), p. 1746-1751por
dc.identifier.doi10.1111/j.1365-2621.2002.tb08717.x
dc.identifier.urihttp://hdl.handle.net/10400.14/6808
dc.language.isoengpor
dc.peerreviewedyespor
dc.publisherWiley-Blackwellpor
dc.subjectMilk proteinspor
dc.subjectThistlepor
dc.subjectFPLCpor
dc.subjectUrea-PAGEpor
dc.titleHydrolysis of caseins by extracts of Cynara Cardunculus precipitated by ammonium sulfatepor
dc.typejournal article
dspace.entity.typePublication
person.familyNameBARROS
person.familyNameMalcata
person.givenNameRUI
person.givenNameFrancisco
person.identifier.ciencia-id111D-8A7B-0ED1
person.identifier.ciencia-id1B13-38A5-35F5
person.identifier.orcid0000-0001-5123-2918
person.identifier.orcid0000-0003-3073-1659
person.identifier.ridJ-3340-2015
person.identifier.scopus-author-id7102542478
rcaap.rightsrestrictedAccesspor
rcaap.typearticlepor
relation.isAuthorOfPublicationa7bdc538-6d0d-4096-9998-a7be5d07e5d6
relation.isAuthorOfPublicationa06c00da-0e2f-4434-8ede-4d7b22c0dfe9
relation.isAuthorOfPublication.latestForDiscoverya06c00da-0e2f-4434-8ede-4d7b22c0dfe9

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