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Antimicrobial resistance and clonal lineages of staphylococcus aureus from cattle: a cross-sectional study from the one health perspective

2022-05Journal article Open access
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dc.contributor.authorSilva, Vanessa
dc.contributor.authorCorreia, Susana
dc.contributor.authorRocha, Jaqueline
dc.contributor.authorManaia, Célia M.
dc.contributor.authorSilva, Adriana
dc.contributor.authorGarcía-Díez, Juan
dc.contributor.authorPereira, José Eduardo
dc.contributor.authorSemedo-Lemsaddek, Teresa
dc.contributor.authorIgrejas, Gilberto
dc.contributor.authorPoeta, Patrícia
dc.date.accessioned2022-05-17T16:06:39Z
dc.date.available2022-05-17T16:06:39Z
dc.date.issued2022-05
dc.description.abstractStaphylococcus aureus have been progressively identified in farm animals and in humans with direct contact with these animals showing that S. aureus may be a major zoonotic pathogen. Therefore, we aimed to isolate S. aureus from cows, their handlers, and their immediate surroundings, and to investigate the antimicrobial resistance and genetic lineages of the isolates. Mouth and nose swabs of 244 healthy cows (195 Maronesa, 11 Holstein-Friesians, and 28 crossbreeds), 82 farm workers, 53 water and 63 soil samples were collected. Identification of species was carried out by MALDI-TOF MS Biotyper. The presence of antimicrobial resistance genes and virulence factors was assessed based on gene search by PCR. All isolates were typed by multilocus sequence typing and spa-typing. From 442 samples, 33 (13.9%), 24 (29.3%), 1 (2%), and 1 (2%) S. aureus were recovered from cows, farm workers, water, and soil samples, respectively. Most of the isolates showed resistance only to penicillin. S. aureus isolates were ascribed to 17 sequence types (STs) and 26 spa-types. Some clonal lineages were common to both cows and farm workers such as ST30-t9413, ST72-t148, and ST45-t350. Through a One Health approach, this study revealed that there is a great diversity of clonal lineages of S. aureus in cows and their handlers. Furthermore, some S. aureus lineages are common to cows and handlers, which may suggest a possible transmission.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.doi10.3390/microorganisms10050941pt_PT
dc.identifier.eid85129212727
dc.identifier.issn2076-2607
dc.identifier.pmcPMC9144820
dc.identifier.pmid35630384
dc.identifier.urihttp://hdl.handle.net/10400.14/37611
dc.identifier.wos000804277200001
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectCattlept_PT
dc.subjectCowspt_PT
dc.subjectStaphylococcus aureuspt_PT
dc.subjectTransmissionpt_PT
dc.titleAntimicrobial resistance and clonal lineages of staphylococcus aureus from cattle: a cross-sectional study from the one health perspectivept_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage14pt_PT
oaire.citation.issue5pt_PT
oaire.citation.startPage1pt_PT
oaire.citation.titleMicroorganismspt_PT
oaire.citation.volume10pt_PT
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT

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