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Rapidly responsive silk fibroin hydrogels as an artificial matrix for the programmed tumor cells death

2018Journal article Open access
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dc.contributor.authorRibeiro, Viviana P.
dc.contributor.authorSilva-Correia, Joana
dc.contributor.authorGonçalves, Cristiana
dc.contributor.authorPina, Sandra
dc.contributor.authorRadhouani, Hajer
dc.contributor.authorMontonen, Toni
dc.contributor.authorOliveira, Joaquim M.
dc.contributor.authorOliveira, Ana L.
dc.date.accessioned2018-09-20T10:55:16Z
dc.date.available2018-09-20T10:55:16Z
dc.date.issued2018
dc.description.abstractTimely and spatially-regulated injectable hydrogels, able to suppress growing tumors in response to conformational transitions of proteins, are of great interest in cancer research and treatment. Herein, we report rapidly responsive silk fibroin (SF) hydrogels formed by a horseradish peroxidase (HRP) crosslinking reaction at physiological conditions, and demonstrate their use as an artificial biomimetic three-dimensional (3D) matrix. The proposed SF hydrogels presented a viscoelastic nature of injectable hydrogels and spontaneous conformational changes from random coil to β-sheet conformation under physiological conditions. A human neuronal glioblastoma (U251) cell line was used for screening cell encapsulation and in vitro evaluation within the SF hydrogels. The transparent random coil SF hydrogels promoted cell viability and proliferation up to 10 days of culturing, while the crystalline SF hydrogels converted into β-sheet structure induced the formation of TUNEL-positive apoptotic cells. Therefore, this work provides a powerful tool for the investigation of the microenvironment on the programed tumor cells death, by using rapidly responsive SF hydrogels as 3D in vitro tumor models.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationRibeiro, V.P., Silva-Correia, J., Gonçalves, C., Pina, S., Radhouani, H., Montonen, T., … Oliveira, J.M. (2018). Rapidly responsive silk fibroin hydrogels as an artificial matrix for the programmed tumor cells death. PLoS ONE 13(4), e0194441pt_PT
dc.identifier.doi10.1371/journal.pone.0194441pt_PT
dc.identifier.eid85045026670
dc.identifier.issn1932-6203
dc.identifier.pmcPMC5884513
dc.identifier.pmid29617395
dc.identifier.urihttp://hdl.handle.net/10400.14/25720
dc.identifier.wos000429203800031
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherPublic Library Science
dc.relationIF/00423/2012
dc.relationPD/59/2013
dc.relationBioengineered Silk Fibroin-Based Contructs with Potential for Osteochondral Tissue Regeneration
dc.relation“Novel biomaterials for treatment of osteoarthritis”
dc.relationNanomaterials with ionic-dopants possessing osteogenic and neurovascular potential for osteochondral tissue engineering
dc.relationNew Perspectives for Osteoarthritis Treatment: Exopolysaccharides from Kefir as Promising Candidates for Viscosupplementation
dc.relationIF/00115/2015
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.titleRapidly responsive silk fibroin hydrogels as an artificial matrix for the programmed tumor cells deathpt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleBioengineered Silk Fibroin-Based Contructs with Potential for Osteochondral Tissue Regeneration
oaire.awardTitle“Novel biomaterials for treatment of osteoarthritis”
oaire.awardTitleNanomaterials with ionic-dopants possessing osteogenic and neurovascular potential for osteochondral tissue engineering
oaire.awardTitleNew Perspectives for Osteoarthritis Treatment: Exopolysaccharides from Kefir as Promising Candidates for Viscosupplementation
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/COMPETE/PEst-C%2FSAU%2FLA0026%2F2013/PT
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oaire.citation.titlePLoS ONEpt_PT
oaire.fundingStreamCOMPETE
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person.familyNameRibeiro
person.familyNameSilva Correia
person.familyNameGonçalves
person.familyNamePina
person.familyNameMontonen
person.familyNameOliveira
person.familyNameOliveira
person.givenNameDr. Viviana
person.givenNameJoana
person.givenNameCristiana
person.givenNameSandra
person.givenNameToni
person.givenNameJoaquim Miguel
person.givenNameAna
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project.funder.identifierhttp://doi.org/10.13039/501100001871
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project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
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