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Modified Pseudomonas agar: new differential medium for the detection/enumeration of Pseudomonas aeruginosa in mineral water

2002Journal article Open access
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dc.contributor.authorRamalho, Rita
dc.contributor.authorCunha, Joaquim
dc.contributor.authorTeixeira, Paula
dc.contributor.authorGibbs, Paul A.
dc.date.accessioned2011-10-22T12:01:51Z
dc.date.available2011-10-22T12:01:51Z
dc.date.issued2002
dc.description.abstractPseudomonas aeruginosa has been implicated as a foodborne and waterborne pathogen and is now considered a primary infectious agent. In the present study, the survival of P. aeruginosa inoculated in mineral water was evaluated by drop counts on Pseudomonas Agar Base (PAB), PAB with CN supplement X107, PAB with cetrimide, PAB with nalidixic acid, and these media with added FeSO4. Initial counts, before starvation, were the same in all media tested. Following this period, P. aeruginosa became sensitive to PAB with added cetrimide. The addition of FeSO4 did not improve the recovery of stressed P. aeruginosa but gave colonies a typical dark brown colour being easily differentiated from other species that can grow at 42 C. The modified Pseudomonas agar medium was also tested with several P. aeruginosa strains, other species of Pseudomonas, and other genera. Only P. aeruginosa strains (pyocyanin positive) produced the typical colonies. Our results demonstrate that Pseudomonas agar with ferrous sulphate, used for the differentiation of P. aeruginosa colonies, and nalidixic acid, used as an inhibitor of Gram-positive bacteria, might be a useful medium for the detection of injured P. aeruginosa in mineral water.por
dc.identifier.citationRAMALHO, Rita - Modified Pseudomonas agar: new differential medium for the detection/enumeration of Pseudomonas aeruginosa in mineral water. Journal of Microbiological Methods.ISSN 0167-7012. Vol. 49 (2002) , p. 69–74por
dc.identifier.urihttp://hdl.handle.net/10400.14/6807
dc.language.isoengpor
dc.peerreviewedyespor
dc.publisherElsevierpor
dc.relation.publisherversiondoi:10.1016/S0167-7012(01)00365-7por
dc.subjectCetrimidepor
dc.subjectMineral waterpor
dc.subjectNalidixic acidpor
dc.subjectP. aeruginosapor
dc.subjectPyocyaninpor
dc.titleModified Pseudomonas agar: new differential medium for the detection/enumeration of Pseudomonas aeruginosa in mineral waterpor
dc.typejournal article
dspace.entity.typePublication
person.familyNameTeixeira
person.familyNameGibbs
person.givenNamePaula
person.givenNamePaul
person.identifier.orcid0000-0002-6296-5137
person.identifier.orcid0000-0002-6580-4363
person.identifier.ridJ-8678-2014
person.identifier.scopus-author-id7005895206
rcaap.rightsopenAccesspor
rcaap.typearticlepor
relation.isAuthorOfPublicationb918d4a2-b9f9-4c56-8318-111625e4d58b
relation.isAuthorOfPublication218d85c8-40f4-42c0-9a47-4e8ad9f72a96
relation.isAuthorOfPublication.latestForDiscovery218d85c8-40f4-42c0-9a47-4e8ad9f72a96

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