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Bacterial community dynamics in a rotating biological contactor treating 2‑fluorophenol‑containing wastewater

2014Journal article Restricted access
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dc.contributor.authorDuque, Anouk F.
dc.contributor.authorBessa, Vânia S.
dc.contributor.authorCastro, Paula M. L.
dc.date.accessioned2014-05-08T14:24:43Z
dc.date.available2014-05-08T14:24:43Z
dc.date.issued2014
dc.description.abstractOne of the main factors affecting the performance of rotating biological contactors (RBC) is the biofilm characteristics. Therefore, a deep understanding of the microbial population dynamics and structure of the biofilm is mandatory if optimization of organic matter and nutrients removal is targeted. This study focused on the effects of organic shock loads of 2-fluorophenol (2-FP) on the microbial diversity present in an RBC biofilm. The RBC was seeded with activated sludge from a conventional wastewater treatment plant and was operated during 496 days. During the first 126 days, the RBC was subjected to intermittent 2-FP shocks of 25 mg l(-1) and no degradation occurred. Therefore, the reactor was subsequently augmented with a 2-FP-degrading strain (FP1). Afterwards, the RBC had a stable performance when subjected to 2-FP shocks up to 50 mg l(-1) and to a starvation period, as indicated by removal of the compound. Denaturing gradient gel electrophoresis (DGGE) revealed large shifts in microbial communities present in the first and fifth stages, although no clear relation between the sample collection time and spatial factor was found. Phylogenetic affiliation of some predominant members was assessed by direct sequencing of correspondent DGGE bands. Affiliations to alpha-, beta- and delta-Proteobacteria were found. Several bacterial strains isolated from the reactor showed capacity for 2-FP degradation. Strain FP1 was successfully recovered from the biofilm by plating and by DGGE, reinforcing that bioaugmentation was successfully achieved.por
dc.description.versioninfo:eu-repo/semantics/publishedVersion
dc.identifier.citationDUQUE, Anouk F. ; BESSA, Vania S. ; CASTRO, Paula M. L. - Bacterial community dynamics in a rotating biological contactor treating 2‑fluorophenol‑containing wastewater. Journal of Industrial Microbiology & Biotechnology. ISSN 1476-5535. Vol. 41, n.º 1 (2014), p. 97-104por
dc.identifier.doi10.1007/s10295-013-1381-4
dc.identifier.eissn1476-5535
dc.identifier.issn1367-5435
dc.identifier.urihttp://hdl.handle.net/10400.14/14325
dc.identifier.wosWOS:000329324700011
dc.language.isoengpor
dc.peerreviewedyespor
dc.publisherSpringerpor
dc.relationStrategic Project - LA 16 - 2011-2012
dc.subjectRotating biological contactor (RBC)por
dc.subject2-Fluorophenol (2-FP)por
dc.subjectMicrobial dynamicspor
dc.subjectDenaturing gradient gel electrophoresis (DGGE)por
dc.titleBacterial community dynamics in a rotating biological contactor treating 2‑fluorophenol‑containing wastewaterpor
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleStrategic Project - LA 16 - 2011-2012
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/SFRH/SFRH%2FBD%2F30771%2F2006/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/3599-PPCDT/PTDC%2FBIO%2F67306%2F2006/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/PEst-OE%2FEQB%2FLA0016%2F2011/PT
oaire.citation.endPage104
oaire.citation.issue1
oaire.citation.startPage97
oaire.citation.titleJournal of Industrial Microbiology and Biotechnology
oaire.citation.volume41
oaire.fundingStreamSFRH
oaire.fundingStream3599-PPCDT
oaire.fundingStream6817 - DCRRNI ID
person.familyNameF. Duque
person.familyNameBessa
person.familyNameCastro
person.givenNameAnouk
person.givenNameVânia
person.givenNamePaula
person.identifier2013444
person.identifier.ciencia-id7819-FA0D-FC04
person.identifier.ciencia-id7C1F-6C72-354A
person.identifier.orcid0000-0002-8360-1404
person.identifier.orcid0000-0002-2676-7184
person.identifier.orcid0000-0001-8841-6606
person.identifier.ridN-5863-2014
person.identifier.ridK-8029-2014
person.identifier.ridM-8241-2013
person.identifier.scopus-author-id36573459300
person.identifier.scopus-author-id35214245200
person.identifier.scopus-author-id7102781782
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsrestrictedAccesspor
rcaap.typearticlepor
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