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Purification, kinetics and spectral characterisation of a new versatile peroxidase from a Bjerkandera Sp. isolate

2006Journal article Open access
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dc.contributor.authorMoreira, Patrícia R.
dc.contributor.authorBouillenne, F.
dc.contributor.authorVara, E. Almeida
dc.contributor.authorMalcata, F. Xavier
dc.contributor.authorFrère, J. M.
dc.contributor.authorDuarte, J. Cardoso
dc.date.accessioned2010-11-09T13:30:14Z
dc.date.available2010-11-09T13:30:14Z
dc.date.issued2006
dc.description.abstractFrom the extracellular fluid of a novel strain of Bjerkandera sp., it was isolated, purified and identified the main enzyme responsible for Remazol Brilliant Blue R dye decolourisation. Such an enzyme is able to oxidise manganese, as well as veratryl alcohol and 2,6-dimethoxyphenol in manganese-independent reactions; hence, it can be included in the new group of versatile peroxidases. The molecular mass of said enzyme is ca. 45 kDa, and the N-terminal amino acid sequence obtained by Edman degradation is VAXPDGVNTA. The enzyme substrate range for oxidation of several phenolic and non-phenolic aromatic compounds was determined and the corresponding Michaelis-Menten kinetic constants calculated. Furthermore, spectrophotometric assays showing the Soret band and allowing observation of band shifts of the enzyme led to the conclusion that Bjerkandera strains may also synthesise at least two different versatile peroxidases, as happens with Pleurotus eryngii.por
dc.identifier.citationMOREIRA, Patrícia R...[et al.] - Purification, kinetics and spectral characterisation of a new versatile peroxidase from a Bjerkandera Sp. isolate. Enzyme and Microbial Technology. ISSN 0141-0229. Vol. 38, n.º 1-2 (2006), p. 28-33por
dc.identifier.doi10.1016/j.enzmictec.2004.12.035
dc.identifier.urihttp://hdl.handle.net/10400.14/3360
dc.language.isoengpor
dc.publisherElsevierpor
dc.subjectEnzymepor
dc.subjectOxidoreductasepor
dc.subjectWhite-rot funguspor
dc.titlePurification, kinetics and spectral characterisation of a new versatile peroxidase from a Bjerkandera Sp. isolatepor
dc.typejournal article
dspace.entity.typePublication
person.familyNameMoreira
person.familyNameMalcata
person.givenNamePatrícia
person.givenNameFrancisco
person.identifier.ciencia-id3A17-0D5A-2C03
person.identifier.ciencia-id1B13-38A5-35F5
person.identifier.orcid0000-0002-0004-851X
person.identifier.orcid0000-0003-3073-1659
person.identifier.scopus-author-id7006510563
person.identifier.scopus-author-id7102542478
rcaap.rightsopenAccesspor
rcaap.typearticlepor
relation.isAuthorOfPublicationcd69b8c0-d44f-46ae-91d2-24a36418f50a
relation.isAuthorOfPublicationa06c00da-0e2f-4434-8ede-4d7b22c0dfe9
relation.isAuthorOfPublication.latestForDiscoverya06c00da-0e2f-4434-8ede-4d7b22c0dfe9

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