Repository logo
 
Publication

Enantioselective biodegradation of pharmaceuticals, alprenolol and propranolol, by an activated sludge inoculum

2013Journal article Restricted access
Simple item page
dc.contributor.authorRibeiro, Ana R.
dc.contributor.authorAfonso, Carlos M.
dc.contributor.authorCastro, Paula M. L.
dc.contributor.authorTritan, Maria E.
dc.date.accessioned2013-05-03T10:08:50Z
dc.date.available2013-05-03T10:08:50Z
dc.date.issued2013
dc.description.abstractBiodegradation of chiral pharmaceuticals in the environment can be enantioselective. Thus quantification of enantiomeric fractions during the biodegradation process is crucial for assessing the fate of chiral pollutants. This work presents the biodegradation of alprenolol and propranolol using an activated sludge inoculum, monitored by a validated enantioselective HPLC method with fluorescence detection. The enantioseparation was optimized using a vancomycin-based chiral stationary phase under polar ionic mode. The method was validated using a minimal salts medium inoculated with activated sludge as matrix. The method was selective and linear in the range of 10–800 ng/ml, with a R2>0.99. The accuracy ranged from 85.0 percent to 103 percent, the recovery ranged from 79.9 percent to 103 percent, and the precision measured by the relative standard deviation (RSD) was <7.18 percent for intra-batch and <5.39 percent for inter-batch assays. The limits of quantification and detection for all enantiomers were 10 ng/ml and 2.5 ng/ml, respectively. The method was successfully applied to follow the biodegradation of the target pharmaceuticals using an activated sludge inoculum during a fifteen days assay. The results indicated slightly higher biodegradation rates for the S-enantiomeric forms of both beta-blockers. The presence of another carbon source maintained the enantioselective degradation pattern while enhancing biodegradation extent up to fourteen percent.por
dc.description.versioninfo:eu-repo/semantics/publishedVersion
dc.identifier.citationRIBEIRO, Ana R... [et al.] - Enantioselective biodegradation of pharmaceuticals, alprenolol and propranolol, by an activated sludge inoculum. Ecotoxicology and Environmental Safety. ISSN 0147-6513. Vol. 87 (2013), p. 108–114por
dc.identifier.doi10.1016/j.ecoenv.2012.10.009
dc.identifier.eissn1090-2414
dc.identifier.issn0147-6513
dc.identifier.urihttp://hdl.handle.net/10400.14/10418
dc.identifier.wosWOS:000312043100014
dc.language.isoengpor
dc.peerreviewedyespor
dc.publisherElsevierpor
dc.relation09-GCQF-CICS-09
dc.relationFLUOROPHARMA-PTDC/EBB-EBI/111699/2009
dc.subjectChiral ecotoxicitypor
dc.subjectEnantioselective biodegradationpor
dc.subjectHPLC-FDpor
dc.subjectChirobiotic CSPpor
dc.subjectBeta-blockerspor
dc.subjectAlprenololpor
dc.subjectPropranololpor
dc.titleEnantioselective biodegradation of pharmaceuticals, alprenolol and propranolol, by an activated sludge inoculumpor
dc.typejournal article
dspace.entity.typePublication
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/SFRH/SFRH%2FBD%2F64999%2F2009/PT
oaire.citation.endPage114
oaire.citation.startPage108
oaire.citation.titleEcotoxicology and Environmental Safety
oaire.citation.volume87
oaire.fundingStreamSFRH
person.familyNameRibeiro
person.familyNameAfonso
person.familyNameCastro
person.familyNameTiritan
person.givenNameAna Rita Lado
person.givenNameCarlos
person.givenNamePaula
person.givenNameMaria Elizabeth
person.identifier903786
person.identifier2013444
person.identifier1310409
person.identifier.ciencia-id421D-DC6E-F6E3
person.identifier.ciencia-id6A10-3565-6158
person.identifier.ciencia-id7C1F-6C72-354A
person.identifier.ciencia-idD915-BFC0-FB3A
person.identifier.orcid0000-0002-4445-5379
person.identifier.orcid0000-0002-5731-5789
person.identifier.orcid0000-0001-8841-6606
person.identifier.orcid0000-0003-3320-730X
person.identifier.ridI-5989-2015
person.identifier.ridM-8241-2013
person.identifier.ridK-9492-2014
person.identifier.scopus-author-id57207918943
person.identifier.scopus-author-id36145043600
person.identifier.scopus-author-id7102781782
person.identifier.scopus-author-id6602890981
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsrestrictedAccesspor
rcaap.typearticlepor
relation.isAuthorOfPublication7264bdc4-7cd4-4a5a-ac3c-fdf1ebabe89b
relation.isAuthorOfPublication181a86a6-86a0-463c-b86e-d4e5669236da
relation.isAuthorOfPublicationf2253992-dc8d-4042-9a0f-597ebcf0a1d6
relation.isAuthorOfPublication1ab8e8f5-cf82-4edd-89d8-46e5cca13e19
relation.isAuthorOfPublication.latestForDiscoveryf2253992-dc8d-4042-9a0f-597ebcf0a1d6
relation.isProjectOfPublicatione0089ebb-5e73-4ef5-88ac-375c496d78c0
relation.isProjectOfPublication.latestForDiscoverye0089ebb-5e73-4ef5-88ac-375c496d78c0

Files

Original bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
19819942.pdf
Size:
470.25 KB
Format:
Adobe Portable Document Format
Download
License bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
3.44 KB
Format:
Item-specific license agreed upon to submission
Description:
Download

Collections

CBQF - Contribuições em Revistas Científicas / Contribution to Journals