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Optimisation, by response surface methodology, of degree of hydrolysis and antioxidant and ACE-inhibitory activities of whey protein hydrolysates obtained with cardoon extract

2011Journal article Restricted access
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dc.contributor.authorTavares, T. G.
dc.contributor.authorContreras, M. M.
dc.contributor.authorAmorim, M.
dc.contributor.authorMartín-Álvarez, P. J.
dc.contributor.authorPintado, M. E.
dc.contributor.authorRecio, I.
dc.contributor.authorMalcata, F. X.
dc.date.accessioned2012-02-27T16:15:31Z
dc.date.available2012-02-27T16:15:31Z
dc.date.issued2011
dc.description.abstractThe hydrolysis of bovine whey protein concentrate (WPC), alpha-lactalbumin (alpha-La) and caseinomacropeptide (CMP), by aqueous extracts of Cynara cardunculus, was optimized using response surface methodology. Degree of hydrolysis (DH), angiotensin-converting enzyme (ACE)-inhibitory activity and antioxidant activity were used as objective functions, and hydrolysis time and enzyme/substrate ratio as manipulated parameters. The model was statistically appropriate to describe ACE-inhibitory activity of hydrolysates from WPC and alpha-La, but not from CMP. Maximum DH was 18% and 9%, for WPC and alpha-La, respectively. 50% ACE-inhibition was produced by 105.4 (total fraction) and 25.6 mu g mL(-1) (<3 kDa fraction) for WPC, and 47.6 (total fraction) and 22.5 mu g mL(-1) (<3 kDa fraction) for alpha-La. Major peptides of fractions exhibiting ACE-inhibition were sequenced. The antioxidant activities of WPC and alpha-La were 0.96 +/- 0.08 and 1.12 +/- 0.13 mmol trolox equivalent per mg hydrolysed protein, respectively.por
dc.description.versioninfo:eu-repo/semantics/publishedVersion
dc.identifier.citationTAVARES, T. G... [et al.] - Optimisation, by response surface methodology, of degree of hydrolysis and antioxidant and ACE-inhibitory activities of whey protein hydrolysates obtained with cardoon extract. International Dairy Journal. ISSN 0958-6946. Vol. 21, n.º 12 (2011), p. 926-933por
dc.identifier.doi10.1016/j.idairyj.2011.05.013
dc.identifier.eid80052907959
dc.identifier.eissn1879-0143
dc.identifier.issn0958-6946
dc.identifier.urihttp://hdl.handle.net/10400.14/7964
dc.identifier.wos000295830900004
dc.language.isoengpor
dc.peerreviewedyespor
dc.publisherElsevierpor
dc.relation2007PT0033
dc.relationFUN-C-Food CSD2007-00063
dc.relationAGL2008-01713, P2009/AGR-1469
dc.titleOptimisation, by response surface methodology, of degree of hydrolysis and antioxidant and ACE-inhibitory activities of whey protein hydrolysates obtained with cardoon extractpor
dc.typejournal article
dspace.entity.typePublication
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/SFRH/SFRH%2FBD%2F31604%2F2006/PT
oaire.citation.endPage933
oaire.citation.issue12
oaire.citation.startPage926
oaire.citation.titleInternational Dairy Journal
oaire.citation.volume21
oaire.fundingStreamSFRH
person.familyNameGranja Tavares
person.familyNameCONTRERAS
person.familyNameAmorim
person.familyNamePintado
person.familyNameRecio
person.familyNameMalcata
person.givenNameTânia Sofia
person.givenNameMARIA DEL MAR
person.givenNameMaria Manuela
person.givenNameMaria Manuela
person.givenNameIsidra
person.givenNameFrancisco
person.identifier362150
person.identifier456608
person.identifier.ciencia-idDB16-AFE8-27BD
person.identifier.ciencia-id791D-E9BA-D432
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person.identifier.ciencia-id1B13-38A5-35F5
person.identifier.orcid0000-0002-6329-0602
person.identifier.orcid0000-0002-3407-0088
person.identifier.orcid0000-0002-1485-0423
person.identifier.orcid0000-0002-0760-3184
person.identifier.orcid0000-0003-2973-5329
person.identifier.orcid0000-0003-3073-1659
person.identifier.ridK-9122-2014
person.identifier.ridL-4327-2014
person.identifier.ridF-5696-2013
person.identifier.ridH-4057-2012
person.identifier.scopus-author-id7003730119
person.identifier.scopus-author-id26653952000
person.identifier.scopus-author-id55293251500
person.identifier.scopus-author-id7004483898
person.identifier.scopus-author-id7102542478
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsrestrictedAccesspor
rcaap.typearticlepor
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