Publication
Efficiency of four secondary enrichment protocols in differentiation and isolation of Listeria spp. and Listeria monocytogenes from smoked fish processing chains
| dc.contributor.author | Duarte, Gabriela | |
| dc.contributor.author | Vaz-Velho, Manuela | |
| dc.contributor.author | Capella, Christopher | |
| dc.contributor.author | Gibbs, Paul | |
| dc.date.accessioned | 2011-10-23T08:44:15Z | |
| dc.date.available | 2011-10-23T08:44:15Z | |
| dc.date.issued | 1999 | |
| dc.description.abstract | Four secondary enrichment protocols (conventional methods: UVM II, Fraser 24 h and Fraser 48 h: Impedimetric method: Listeria electrical detection medium) were studied for their ability to isolate Listeria spp. and Listeria monocytogenes from fish and environmental samples collected along the processing chain of cold-smoked fish. From all methods, Listeria spp. and L. monocytogenes were respectively present in 56 and 34 of 315 samples analysed. Fraser broth incubated for 48 h gave the fewest false negative Listeria spp. results [4/56; (7.1%)], but concurrently only 15/34 (44.1%) samples were correctly identified as containing L. monocytogenes. Listeria electrical detection (LED) medium detected only 36/56 (64.3%) Listeria spp. positive samples. Despite this lower isolation rate, LED identified 20/34 (58.8%) L. monocytogenes positive samples correctly and gave fewer false positive results. The overall conclusion was that more than one isolation method is needed to accurately estimate L. monocytogenes contamination rates. | por |
| dc.identifier.citation | DUARTE, Gabriela... [et al.] - Efficiency of four secondary enrichment protocols in differentiation and isolation of Listeria spp. and Listeria monocytogenes from smoked fish processing chains. International Journal of Food Microbiology. Vol. 52, n.º 3 (1999), p. 163–168 | por |
| dc.identifier.doi | 10.1016/S0168-1605(99)00121-X | |
| dc.identifier.uri | http://hdl.handle.net/10400.14/6908 | |
| dc.language.iso | eng | por |
| dc.peerreviewed | yes | por |
| dc.publisher | Elsevier | por |
| dc.subject | Cold-smoked fish | por |
| dc.subject | Listeria | por |
| dc.subject | Listeria monocytogenes | por |
| dc.subject | Secondary selective enrichments | por |
| dc.subject | Conventional cultural and bactometer capacitance-based detection methods | por |
| dc.title | Efficiency of four secondary enrichment protocols in differentiation and isolation of Listeria spp. and Listeria monocytogenes from smoked fish processing chains | por |
| dc.type | journal article | |
| dspace.entity.type | Publication | |
| person.familyName | Vaz Velho | |
| person.familyName | Gibbs | |
| person.givenName | Manuela | |
| person.givenName | Paul | |
| person.identifier.ciencia-id | C719-383D-5548 | |
| person.identifier.orcid | 0000-0003-4825-2058 | |
| person.identifier.orcid | 0000-0002-6580-4363 | |
| person.identifier.rid | K-3058-2014 | |
| person.identifier.scopus-author-id | 55999738500 | |
| rcaap.rights | openAccess | por |
| rcaap.type | article | por |
| relation.isAuthorOfPublication | ceca7b30-6960-4ad2-bb0c-675fcca89f86 | |
| relation.isAuthorOfPublication | 218d85c8-40f4-42c0-9a47-4e8ad9f72a96 | |
| relation.isAuthorOfPublication.latestForDiscovery | 218d85c8-40f4-42c0-9a47-4e8ad9f72a96 |
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