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Advisor(s)
Abstract(s)
Aims: The present work aimed to characterize the impact of an anthocyaninrich
blueberry extract upon the growth, adhesion and biofilm formation of
several pathogens including some multiresistant bacteria.
Methods and Results: A group comprised of reference strains and clinical
multiresistant isolates of Pseudomonas aeruginosa, Escherichia coli, Proteus
mirabilis, Acinetobacter baumannii and Staphylococcus aureus, were used to
screen for antimicrobial activity. Microbial growth was determined through the
measurement of the optical density while adhesion and biofilm formation was
determined using the standard crystal violet staining procedure. The results
showed that, while blueberry extract was only effective in hindering the growth
of Staph. aureus and E. coli, it was capable of significantly inhibiting biofilm
formation and bacterial adhesion for all micro-organisms tested.
Conclusions: The extract demonstrated a considerable potential as a natural,
alternative antimicrobial capable of either interfering with microbial growth or
hamper the adhesion to surfaces, with Staph. aureus proving to be the most
susceptible micro-organism.
Significance and Impact of the Study: The overall study demonstrates the
potential of anthocyanin extracts as natural effective alternative antimicrobial
agents. Additionally, the extract’s capacity to reduce adhesion without reducing
bacterial growth reduces the likeliness of resistance development while reducing
the probability of infection.
Description
Keywords
Adhesion inhibition Anthocyanins Antibiofilm activity Antimicrobial activity Blueberry Vaccinium corymbosum
Citation
SILVA, Sara; COSTA, Eduardo M.; MENDES, M.; MORAIS, R. M. S. C.; CALHAU, Conceição; PINTADO, M. E. - Antimicrobial, antiadhesive and antibiofilm activity of an ethanolic, anthocyanin-rich blueberry extract purified by solid phase extraction. Journal of Applied Microbiology. ISSN 1365-2672. Vol. 121, n.º 3 (2016), p. 693-703
Publisher
Wiley