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Preservation of human gut microbiota inoculums for in vitro fermentations studies

2021-03Journal article Open access
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dc.contributor.authorCarvalho, Nelson Mota de
dc.contributor.authorOliveira, Diana Luazi
dc.contributor.authorSaleh, Mayra Anton Dib
dc.contributor.authorPintado, Manuela
dc.contributor.authorMadureira, Ana Raquel
dc.date.accessioned2021-05-24T13:34:30Z
dc.date.available2021-05-24T13:34:30Z
dc.date.issued2021-03
dc.description.abstractThe use of fecal inoculums for in vitro fermentation models requires a viable gut microbiota, capable of fermenting the unabsorbed nutrients. Fresh samples from human donors are used; however, the availability of fresh fecal inoculum and its inherent variability is often a problem. This study aimed to optimize a method of preserving pooled human fecal samples for in vitro fermentation studies. Different conditions and times of storage at -20 degrees C were tested. In vitro fermentation experiments were carried out for both fresh and frozen inoculums, and the metabolic profile compared. In comparison with the fresh, the inoculum frozen in a PBS and 30% glycerol solution, had a significantly lower (p < 0.05) bacterial count (<1 log CFU/mL). However, no significant differences (p < 0.05) were found between the metabolic profiles after 48 h. Hence, a PBS and 30% glycerol solution can be used to maintain the gut microbiota viability during storage at -20 degrees C for at least 3 months, without interfering with the normal course of colonic fermentation.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.doi10.3390/fermentation7010014pt_PT
dc.identifier.eid85106961632
dc.identifier.issn2311-5637
dc.identifier.urihttp://hdl.handle.net/10400.14/33189
dc.identifier.wos000633057500001
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectGut microbiota viabilitypt_PT
dc.subjectGlycerolpt_PT
dc.subjectHuman in vitro modelspt_PT
dc.subjectColon fermentationpt_PT
dc.subjectOrganic acidspt_PT
dc.subjectPreservationpt_PT
dc.titlePreservation of human gut microbiota inoculums for in vitro fermentations studiespt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.issue1pt_PT
oaire.citation.titleFermentationpt_PT
oaire.citation.volume7pt_PT
person.familyNameOliveira
person.familyNameA. D. Saleh
person.familyNamePintado
person.familyNameMadureira
person.givenNameDiana
person.givenNameMayra
person.givenNameMaria Manuela
person.givenNameRaquel
person.identifier1415351
person.identifier456608
person.identifierM-4659-2013
person.identifier.ciencia-id9510-5561-CD65
person.identifier.ciencia-id2F13-AAE0-3405
person.identifier.ciencia-id1C14-25F8-CC07
person.identifier.orcid0000-0003-1412-0655
person.identifier.orcid0000-0002-2334-3626
person.identifier.orcid0000-0002-0760-3184
person.identifier.orcid0000-0001-6596-1927
person.identifier.ridL-8935-2016
person.identifier.ridH-1261-2014
person.identifier.ridF-5696-2013
person.identifier.ridM-4659-2013
person.identifier.scopus-author-id7004483898
person.identifier.scopus-author-id8384097900
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublication43d5a1d2-0fd6-4ee1-a4fa-3d262a8d1cba
relation.isAuthorOfPublicationceb0974a-e4e4-4704-8c98-745ab0c390e7
relation.isAuthorOfPublicationba387c7d-27c9-4016-895c-b35597e91ebc
relation.isAuthorOfPublicationd33a1d99-423e-4ecd-ae9e-b3e46262b521
relation.isAuthorOfPublication.latestForDiscoveryba387c7d-27c9-4016-895c-b35597e91ebc

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