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Potential of omega-3 and conjugated fatty acids to control microglia inflammatory imbalance elicited by obesogenic nutrients

2023-07-01Journal article Open access
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dc.contributor.authorSalsinha, A. S.
dc.contributor.authorSocodato, R.
dc.contributor.authorRodrigues, A.
dc.contributor.authorVale-Silva, R.
dc.contributor.authorRelvas, J. B.
dc.contributor.authorPintado, M.
dc.contributor.authorRodríguez-Alcalá, L. M.
dc.date.accessioned2023-05-18T15:34:21Z
dc.date.available2023-05-18T15:34:21Z
dc.date.issued2023-07-01
dc.description.abstractHigh-fat diet-induced obesity detrimentally affects brain function by inducing chronic low-grade inflammation. This neuroinflammation is, at least in part, likely to be mediated by microglia, which are the main immune cell population in the brain. Microglia express a wide range of lipid-sensitive receptors and their activity can be modulated by fatty acids that cross the blood-brain barrier. Here, by combining live cell imaging and FRET technology we assessed how different fatty acids modulate microglia activity. We demonstrate that the combined action of fructose and palmitic acid induce Ikβα degradation and nuclear translocation of the p65 subunit nuclear factor kB (NF-κB) in HCM3 human microglia. Such obesogenic nutrients also lead to reactive oxygen species production and LynSrc activation (critical regulators of microglia inflammation). Importantly, short-time exposure to omega-3 (EPA and DHA), CLA and CLNA are sufficient to abolish NF-κB pathway activation, suggesting a potential neuroprotective role. Omega-3 and CLA also show an antioxidant potential by inhibiting reactive oxygen species production, and the activation of LynSrc in microglia. Furthermore, using chemical agonists (TUG-891) and antagonists (AH7614) of GPR120/FFA4, we demonstrated that omega-3, CLA and CLNA inhibition of the NF-κB pathway is mediated by this receptor, while omega-3 and CLA antioxidant potential occurs through different signaling mechanisms.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.doi10.1016/j.bbalip.2023.159331pt_PT
dc.identifier.eid85159312987
dc.identifier.issn1388-1981
dc.identifier.pmid37172801
dc.identifier.urihttp://hdl.handle.net/10400.14/41171
dc.identifier.wos001002003500001
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/pt_PT
dc.subjectMicroglia activationpt_PT
dc.subjectOmega-3 fatty acidspt_PT
dc.subjectConjugated fatty acidspt_PT
dc.subjectObesitypt_PT
dc.subjectPalmitic acidpt_PT
dc.subjectFructosept_PT
dc.titlePotential of omega-3 and conjugated fatty acids to control microglia inflammatory imbalance elicited by obesogenic nutrientspt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.issue7
oaire.citation.titleBiochimica et Biophysica Acta - Molecular and Cell Biology of Lipidspt_PT
oaire.citation.volume1868
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT

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