Repository logo
 
Publication

Combined use of chitosan and olfactory mucosa mesenchymal stem/stromal cells to promote peripheral nerve regeneration in vivo

2021Journal article Open access
Simple item page
dc.contributor.authorAlvites, Rui D.
dc.contributor.authorBranquinho, Mariana V.
dc.contributor.authorSousa, Ana C.
dc.contributor.authorAmorim, Irina
dc.contributor.authorMagalhães, Rui
dc.contributor.authorJoão, Filipa
dc.contributor.authorAlmeida, Diogo
dc.contributor.authorAmado, Sandra
dc.contributor.authorPrada, Justina
dc.contributor.authorPires, Isabel
dc.contributor.authorZen, Federica
dc.contributor.authorRaimondo, Stefania
dc.contributor.authorLuís, Ana L.
dc.contributor.authorGeuna, Stefano
dc.contributor.authorVarejão, Artur S. P.
dc.contributor.authorMaurício, Ana C.
dc.date.accessioned2021-02-03T14:47:34Z
dc.date.available2021-02-03T14:47:34Z
dc.date.issued2021
dc.description.abstractPeripheral nerve injury remains a clinical challenge with severe physiological and functional consequences. Despite the existence of multiple possible therapeutic approaches, until now, there is no consensus regarding the advantages of each option or the best methodology in promoting nerve regeneration. Regenerative medicine is a promise to overcome this medical limitation, and in this work, chitosan nerve guide conduits and olfactory mucosa mesenchymal stem/stromal cells were applied in different therapeutic combinations to promote regeneration in sciatic nerves after neurotmesis injury. Over 20 weeks, the intervened animals were subjected to a regular functional assessment (determination of motor performance, nociception, and sciatic indexes), and after this period, they were evaluated kinematically and the sciatic nerves and cranial tibial muscles were evaluated stereologically and histomorphometrically, respectively. The results obtained allowed confirming the beneficial effects of using these therapeutic approaches. The use of chitosan NGCs and cells resulted in better motor performance, better sciatic indexes, and lower gait dysfunction after 20 weeks. The use of only NGGs demonstrated better nociceptive recoveries. The stereological evaluation of the sciatic nerve revealed identical values in the different parameters for all therapeutic groups. In the muscle histomorphometric evaluation, the groups treated with NGCs and cells showed results close to those of the group that received traditional sutures, the one with the best final values. The therapeutic combinations studied show promising outcomes and should be the target of new future works to overcome some irregularities found in the results and establish the combination of nerve guidance conduits and olfactory mucosa mesenchymal stem/stromal cells as viable options in the treatment of peripheral nerves after injury.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationAlvites, R. D., Branquinho, M. V., Sousa, A. C., Amorim, I., Magalhães, R., João, F., ... Maurício, A. C. (2021). Combined use of chitosan and olfactory mucosa mesenchymal stem/stromal cells to promote peripheral nerve regeneration in vivo. Stem Cells Internationalpt_PT
dc.identifier.doi10.1155/2021/6613029pt_PT
dc.identifier.eid85100212122
dc.identifier.eissn1687-9678
dc.identifier.issn1687-966X
dc.identifier.pmcPMC7801080
dc.identifier.pmid33488738
dc.identifier.urihttp://hdl.handle.net/10400.14/31882
dc.identifier.wos000609622700001
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherHindawipt_PT
dc.relationPEst-OE/AGR/ UI0211/2011pt_PT
dc.relationPOCI-01-0247-FEDER-017771pt_PT
dc.relationPOCI-01-0247-FEDER-033877pt_PT
dc.relationPOCI-01-0145-FEDER-031146pt_PT
dc.relationRegenerative therapies applying synovial membrane-derived mesenchymal stem cells targeting the musculoskeletal system
dc.relationAssociation of 3D printing and cellular therapies for regenerative medicine
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.titleCombined use of chitosan and olfactory mucosa mesenchymal stem/stromal cells to promote peripheral nerve regeneration in vivopt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleRegenerative therapies applying synovial membrane-derived mesenchymal stem cells targeting the musculoskeletal system
oaire.awardTitleAssociation of 3D printing and cellular therapies for regenerative medicine
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/POR_NORTE/SFRH%2FBD%2F146172%2F2019/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT//SFRH%2FBD%2F146689%2F2019/PT
oaire.citation.titleStem Cells Internationalpt_PT
oaire.fundingStreamPOR_NORTE
person.familyNameMagalhães
person.familyNameMaurício
person.givenNameRui
person.givenNameAna Colette
person.identifierK-1359-2014
person.identifier.ciencia-id4C1A-BDD4-D716
person.identifier.ciencia-id901B-F8EB-BD8D
person.identifier.orcid0000-0003-3172-440X
person.identifier.orcid0000-0002-0018-9363
person.identifier.ridK-1359-2014
person.identifier.ridE-8917-2013
person.identifier.scopus-author-id6603813414
person.identifier.scopus-author-id12143945000
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublicationb0b10107-23c3-4029-9373-3865df284308
relation.isAuthorOfPublicationc7b82486-6287-41a8-a74a-274ca660626b
relation.isAuthorOfPublication.latestForDiscoveryb0b10107-23c3-4029-9373-3865df284308
relation.isProjectOfPublicatione5edeba8-85e3-440a-a1f1-5ba97f170282
relation.isProjectOfPublicationd8e5b6d4-7aea-4785-8798-7be6e1db75b3
relation.isProjectOfPublication.latestForDiscoveryd8e5b6d4-7aea-4785-8798-7be6e1db75b3

Files

Original bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
28059566.pdf
Size:
9.14 MB
Format:
Adobe Portable Document Format
Download

Collections

CBQF - Contribuições em Revistas Científicas / Contribution to Journals