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Membrane fractionation of Cynara cardunculus swine blood hydrolysate: ingredients of high nutritional and nutraceutical value

2022-08Journal article Open access
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dc.contributor.authorAraújo-Rodrigues, Helena
dc.contributor.authorCoscueta, Ezequiel R.
dc.contributor.authorPereira, Miguel F.
dc.contributor.authorCunha, Sara A.
dc.contributor.authorAlmeida, André
dc.contributor.authorRosa, Ana
dc.contributor.authorMartins, Rui
dc.contributor.authorPereira, Carlos D.
dc.contributor.authorPintado, Manuela E.
dc.date.accessioned2022-07-07T15:34:31Z
dc.date.available2023-08-01T00:30:38Z
dc.date.issued2022-08
dc.description.abstractThis work proposes an innovative approach to valorise swine blood based on enzymatic hydrolysis and membrane fractionations. Hydrolysis with Cynara cardunculus enzymes, followed by microfiltration and double nanofiltration generated three high protein fractions, retentate of microfiltration (RMF; >0.5 µm) and retentate of nanofiltration (RNF; >3 kDa) with approximately 90% of protein on a dry basis and filtrate of nanofiltrate (FNF; <3 kDa) with 65%. FNF, rich in low molecular weight peptides, showed excellent antioxidants (ABTS and ORAC of 911.81 and 532.82 µmol TE g−1 DB, respectively) and antihypertensive (IC50 of 28.51 µg mL−1) potential. By peptidomics and in silico analysis, 43 unique sequences of interest were found, among which LVV-Hemorphin-7 was identified. This hemorphin was demonstrated as the main responsible for the observed bioactivity. Complementary results showed a prebiotic effect mainly for the growth of Bifidobacterium animalis Bo, as well as interesting free amino acids (mainly glutamic acid, leucine, alanine, phenylalanine and aspartic acid) and mineral (e.g., Ca, Mg, P, K and Na) profiles. No antibacterial effect was verified for the seven pathogenic bacteria tested. This study allowed obtaining new ingredient of high nutritional and nutraceutical value for human consumption, with a perspective of sustainability and industrial viability.pt_PT
dc.description.versioninfo:eu-repo/semantics/acceptedVersionpt_PT
dc.identifier.doi10.1016/j.foodres.2022.111549pt_PT
dc.identifier.eid85132907959
dc.identifier.issn0963-9969
dc.identifier.pmid35840243
dc.identifier.urihttp://hdl.handle.net/10400.14/38115
dc.identifier.wos000826360000001
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.subjectACE inhibitorpt_PT
dc.subjectAntioxidant activitypt_PT
dc.subjectBioactive peptidespt_PT
dc.subjectBIOPEPpt_PT
dc.subjectEnzymatic hydrolysispt_PT
dc.subjectFunctional ingredientpt_PT
dc.subjectSwine bloodpt_PT
dc.subjectWaste valorisationpt_PT
dc.titleMembrane fractionation of Cynara cardunculus swine blood hydrolysate: ingredients of high nutritional and nutraceutical valuept_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage11pt_PT
oaire.citation.startPage1pt_PT
oaire.citation.titleFood Research Internationalpt_PT
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT

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