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Chitosan-based nanoparticles for rosmarinic acid ocular delivery-in vitro tests

2016Journal article Open access
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dc.contributor.authorSilva, Sara
dc.contributor.authorFerreira, Domingos
dc.contributor.authorPintado, M. E.
dc.contributor.authorSarmento, Bruno
dc.date.accessioned2017-10-13T08:22:38Z
dc.date.available2017-10-13T08:22:38Z
dc.date.issued2016
dc.description.abstracttIn this study, chitosan nanoparticles were used to encapsulate antioxidant rosmarinic acid, Salvia offici-nalis (sage) and Satureja montana (savory) extracts as rosmarinic acid natural vehicles. The nanoparticleswere prepared by ionic gelation using chitosan and sodium tripolyphosphate (TPP) in a mass ratio of7:1, at pH 5.8. Particle size distribution analysis and transmission electron microscopy (TEM) confirmedthe size ranging from 200 to 300 nm, while surface charge of nanoparticles ranged from 20 to 30 mV.Nanoparticles demonstrate to be safe without relevant cytotoxicity against retina pigment epithelium(ARPE-19) and human cornea cell line (HCE-T). The permeability study in HCE monolayer cell line showedan apparent permeability coefficient Pappof 3.41 ± 0.99 × 10−5and 3.24 ± 0.79 × 10−5cm/s for rosmarinicacid loaded chitosan nanoparticles and free in solution, respectively. In ARPE-19 monolayer cell line thePappwas 3.39 ± 0.18 × 10−5and 3.60 ± 0.05 × 10−5cm/s for rosmarinic acid loaded chitosan nanoparti-cles and free in solution, respectively. Considering the mucin interaction method, nanoparticles indicatemucoadhesive proprieties suggesting an increased retention time over the ocular mucosa after instilla-tion. These nanoparticles may be promising drug delivery systems for ocular application in oxidative eyeconditions.pt_PT
dc.description.versioninfo:eu-repo/semantics/acceptedVersionpt_PT
dc.identifier.citationSILVA, Sara; FERREIRA, Domingos; PINTADO, M. E.; SARMENTO, Bruno - Chitosan-based nanoparticles for rosmarinic acid ocular delivery-in vitro tests. International Journal of Biological Macromolecules. ISSN 0141-8130. Vol. 84 (2016), p. 112-120pt_PT
dc.identifier.doi10.1016/j.ijbiomac.2015.11.070pt_PT
dc.identifier.eid84950272691
dc.identifier.pmid26645149
dc.identifier.urihttp://hdl.handle.net/10400.14/23071
dc.identifier.wos000369456800016
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherElsevier
dc.relationANTIOXIDANT CONTAINING CHITOSAN NANOMEDICINES FOR THE PREVENTION AND TREATMENT OF DIABETIC RETINOPATHY
dc.subjectChitosanpt_PT
dc.subjectNanoparticlespt_PT
dc.subjectRosmarinic acidpt_PT
dc.subjectCell modelspt_PT
dc.titleChitosan-based nanoparticles for rosmarinic acid ocular delivery-in vitro testspt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleANTIOXIDANT CONTAINING CHITOSAN NANOMEDICINES FOR THE PREVENTION AND TREATMENT OF DIABETIC RETINOPATHY
oaire.awardURIinfo:eu-repo/grantAgreement/FCT//SFRH%2FBD%2F61423%2F2009/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/3599-PPCDT/PEst-OE%2FEQB%2FLA0016%2F2013/PT
oaire.citation.endPage120
oaire.citation.startPage112
oaire.citation.titleInternational Journal of Biological Macromoleculespt_PT
oaire.citation.volume84
oaire.fundingStream3599-PPCDT
person.familyNameBaptista da Silva
person.familyNameFerreira
person.familyNamePintado
person.familyNameSarmento
person.givenNameSara
person.givenNameDomingos
person.givenNameMaria Manuela
person.givenNameBruno
person.identifier404100
person.identifier456608
person.identifier152609
person.identifier.ciencia-idC510-41A8-08DC
person.identifier.ciencia-id1C18-A963-BC5E
person.identifier.ciencia-id2F13-AAE0-3405
person.identifier.ciencia-idE816-B6B0-06A5
person.identifier.orcid0000-0003-1805-144X
person.identifier.orcid0000-0001-6200-7169
person.identifier.orcid0000-0002-0760-3184
person.identifier.orcid0000-0001-5763-7553
person.identifier.ridM-7978-2017
person.identifier.ridF-5696-2013
person.identifier.ridJ-6265-2013
person.identifier.scopus-author-id50060987900
person.identifier.scopus-author-id56871285000
person.identifier.scopus-author-id7004483898
person.identifier.scopus-author-id57194334236
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublication404465f0-f877-416f-8259-a3800428580d
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