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Wool-associated proteolytic bacteria, isolated from Portuguese Merino breed

2013Journal article Restricted access
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dc.contributor.authorQueiroga, A. Catarina
dc.contributor.authorPintado, Manuela E.
dc.contributor.authorMalcata, F. Xavier
dc.date.accessioned2013-01-28T14:00:17Z
dc.date.available2013-01-28T14:00:17Z
dc.date.issued2013
dc.description.abstractThe main purpose of this study was to isolate and briefly characterize proteolytic bacteria from a poorly known habitat - raw wool. Fleece samples were accordingly collected from Merino raw wool - a Portuguese ewe breed, at three distinct areas of their body, from animals exhibiting no symptoms or signs of abnormalities: they were then subjected to enumeration and isolation of a total of 158 bacterial strains. Said isolates were screened for protease activity, using the spot technique, on Calcium Caseinate Agar containing 1% (w/v) skim milk. The 36 isolates displaying the highest protease activity underwent a more refined assessment of enzymatic performance - by examining their cell-free supernatant extracts, using casein as substrate. Two Bacillus isolates were eventually chosen owing to their highest proteolytic activities (24.6 and 15.9 U/mL), and identified using molecular biology tools.por
dc.description.versioninfo:eu-repo/semantics/publishedVersion
dc.identifier.citationQUEIROGA, A. Catarina; PINTADO, M. E.; MALCATA, F. Xavier - Wool-associated proteolytic bacteria, isolated from Portuguese Merino breed. Small Ruminant Research. ISSN 0921-4488. Vol. 109, n.º 1 (2013), p. 38–46por
dc.identifier.doi10.1016/j.smallrumres.2012.09.005
dc.identifier.eissn1879-0941
dc.identifier.issn0921-4488
dc.identifier.urihttp://hdl.handle.net/10400.14/9997
dc.identifier.wosWOS:000312509900007
dc.language.isoengpor
dc.peerreviewedyespor
dc.publisherElsevierpor
dc.subjectSheeppor
dc.subjectFleecepor
dc.subjectWool microflorapor
dc.subjectKeratinpor
dc.subjectCaseinolytic activitypor
dc.titleWool-associated proteolytic bacteria, isolated from Portuguese Merino breedpor
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage46
oaire.citation.issue1
oaire.citation.startPage38
oaire.citation.volume109
person.familyNameQueiroga
person.familyNamePintado
person.familyNameMalcata
person.givenNameAna Catarina
person.givenNameMaria Manuela
person.givenNameFrancisco
person.identifier456608
person.identifier.ciencia-id2F13-AAE0-3405
person.identifier.ciencia-id1B13-38A5-35F5
person.identifier.orcid0000-0001-8395-1837
person.identifier.orcid0000-0002-0760-3184
person.identifier.orcid0000-0003-3073-1659
person.identifier.ridJ-7815-2012
person.identifier.ridF-5696-2013
person.identifier.scopus-author-id16067735500
person.identifier.scopus-author-id7004483898
person.identifier.scopus-author-id7102542478
rcaap.rightsrestrictedAccesspor
rcaap.typearticlepor
relation.isAuthorOfPublication42dbfde5-2976-48f9-8423-83d788c1a6aa
relation.isAuthorOfPublicationba387c7d-27c9-4016-895c-b35597e91ebc
relation.isAuthorOfPublicationa06c00da-0e2f-4434-8ede-4d7b22c0dfe9
relation.isAuthorOfPublication.latestForDiscoveryba387c7d-27c9-4016-895c-b35597e91ebc

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