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Detailed kinetic model describing new oligosaccharides synthesis using different β-galactosidases

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dc.contributor.authorRodriguez-Fernandez, Maria
dc.contributor.authorCardelle-Cobas, Alejandra
dc.contributor.authorVillamiel, Mar
dc.contributor.authorBanga, Julio R.
dc.date.accessioned2012-01-16T10:32:53Z
dc.date.available2012-01-16T10:32:53Z
dc.date.issued2011
dc.description.abstractThe production of prebiotic galactooligosaccharides (GOS) from lactose has been widely studied whereas the synthesis of new prebiotic oligosaccharides with improved properties as those derived from lactulose is receiving an increasing interest. Understanding the mechanism of enzymatic oligosaccharides synthesis from lactulose would help to improve the quality of the products in a rational way as well as to increase the production efficiency by optimally selecting the operating conditions. A detailed kinetic model describing the enzymatic transgalactosylation reaction during lactulose hydrolysis is presented here for the first time. The model was calibrated with the experimental data obtained in batch assays with two different B-galactosidases at various temperatures and concentrations of substrate. A complete system identification loop, including model selection, robust estimation of the parameters by means of a global optimization method and computation of confidence intervals was performed. The kinetic model showed a good agreement between experimental data and predictions for lactulose conversion and provided important insights into the mechanism of formation of new gligosaccharides with potential prebiotic properties.por
dc.description.versioninfo:eu-repo/semantics/acceptedVersion
dc.identifier.citationRODRIGUEZ-FERNANDEZ, Maria.. [et al.] - Detailed kinetic model describing new oligosaccharides synthesis using different β-galactosidases. Journal of Biotechnology. ISSN 0168-1656. Vol. 153, n.º 3-4 (2011), p. 116–124por
dc.identifier.doi10.1016/j.jbiotec.2011.03.012
dc.identifier.eissn1873-4863
dc.identifier.issn0168-1656
dc.identifier.urihttp://hdl.handle.net/10400.14/7470
dc.identifier.wosWOS:000290513700006
dc.language.isoengpor
dc.peerreviewedyespor
dc.publisherElsevierpor
dc.relationCSD2007-00063
dc.relationDPI2008-06880-C03-02
dc.relationMEC GEN2006-27747-E/SYS
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/4.0/
dc.subjectKinetic modelspor
dc.subjectLactulosepor
dc.subjectTransgalactosylationpor
dc.subjectModel selectionpor
dc.subjectParameter estimationpor
dc.subjectIdentifiability analysispor
dc.titleDetailed kinetic model describing new oligosaccharides synthesis using different β-galactosidasespor
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage124
oaire.citation.issue3-4
oaire.citation.startPage116
oaire.citation.titleJournal of Biotechnology
oaire.citation.volume153
person.familyNameRodriguez-Fernandez
person.familyNameCardelle Cobas
person.familyNameVillamiel
person.familyNameR. Banga
person.givenNameMaria
person.givenNameAlejandra
person.givenNameMar
person.givenNameJulio
person.identifier282727
person.identifier.orcid0000-0003-1966-2920
person.identifier.orcid0000-0002-1271-513X
person.identifier.orcid0000-0001-6847-8359
person.identifier.orcid0000-0002-4245-0320
person.identifier.ridI-7193-2016
person.identifier.ridK-7183-2017
person.identifier.ridA-8388-2008
person.identifier.scopus-author-id9736687700
person.identifier.scopus-author-id7003744305
person.identifier.scopus-author-id7102475138
rcaap.rightsopenAccesspor
rcaap.typearticlepor
relation.isAuthorOfPublicationdc2c5fe1-76c1-46ba-a334-a4a3c97add52
relation.isAuthorOfPublication48ad3ed1-3a70-42fa-b935-e8ef001f6cce
relation.isAuthorOfPublication31fb2798-cb78-4395-b4ce-b26987947f02
relation.isAuthorOfPublicationf9a75c87-9088-4e58-b507-e76e94d24e0f
relation.isAuthorOfPublication.latestForDiscovery48ad3ed1-3a70-42fa-b935-e8ef001f6cce

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