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Development and characterization of chitosan microparticles-in-films for buccal delivery of bioactive peptides

2019Journal article Open access
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dc.contributor.authorBatista, Patrícia
dc.contributor.authorCastro, Pedro
dc.contributor.authorMadureira, Ana Raquel
dc.contributor.authorSarmento, Bruno
dc.contributor.authorPintado, Manuela
dc.date.accessioned2019-03-12T16:42:47Z
dc.date.available2019-03-12T16:42:47Z
dc.date.issued2019
dc.description.abstractNowadays, bioactive peptides are used for therapeutic applications and the selection of a carrier to deliver them is very important to increase the efficiency, absorption, release, bioavailability and consumer acceptance. The aim of this study was to develop and characterize chitosan-based films loaded with chitosan microparticles containing a bioactive peptide (sequence: KGYGGVSLPEW) with antihypertensive properties. Films were prepared by the solvent casting method, while the microparticles were prepared by ionic gelation. The final optimized chitosan microparticles exhibited a mean diameter of 2.5 m, a polydispersity index of 0.46, a zeta potential of +61 mV and a peptide association efficiency of 76%. Chitosan films were optimized achieving the final formulation of 0.79% (w/v) of chitosan, 6.74% (w/v) of sorbitol and 0.82% (w/v) of citric acid. These thin ( 0.100 mm) and transparent films demonstrated good performance in terms of mechanical and biological properties. The oral films developed were flexible, elastic, easy to handle and exhibited rapid disintegration (30 s) and an erosion behavior of 20% when they came into contact with saliva solution. The cell viability (75–99%) was proved by methylthiazolydiphenyl-tetrazolium bromide (MTT) assay with TR146 cells. The chitosan mucoadhesive films loaded with peptide–chitosan microparticles resulted in an innovative approach to perform administration across the buccal mucosa, because these films present a larger surface area, leading to the rapid disintegration and release of the antihypertensive peptide under controlled conditions in the buccal cavity, thus promoting bioavailability.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationBatista, P., Castro, P., Madureira, A., Sarmento, B., & Pintado, M. (2019). Development and Characterization of Chitosan Microparticles-in-Films for Buccal Delivery of Bioactive Peptides. Pharmaceuticals, 12(1), 32. https://doi.org/10.3390/ph12010032pt_PT
dc.identifier.doi10.3390/ph12010032pt_PT
dc.identifier.eid85064350500
dc.identifier.issn1424-8247
dc.identifier.pmcPMC6469171
dc.identifier.pmid30791572
dc.identifier.urihttp://hdl.handle.net/10400.14/27077
dc.identifier.wos000464181700002
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherMDPIpt_PT
dc.relationDevelopment of oral strips containing nanoencapsulated bioactive compounds
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectBioactive peptidespt_PT
dc.subjectBuccal deliverypt_PT
dc.subjectChitosanpt_PT
dc.subjectMicroparticlespt_PT
dc.subjectOral filmspt_PT
dc.titleDevelopment and characterization of chitosan microparticles-in-films for buccal delivery of bioactive peptidespt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleDevelopment of oral strips containing nanoencapsulated bioactive compounds
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/3599-PPCDT/PTDC%2FBBB-NAN%2F3249%2F2014/PT
oaire.citation.issue1
oaire.citation.titlePharmaceuticalspt_PT
oaire.citation.volume12
oaire.fundingStream3599-PPCDT
person.familyNameBatista
person.familyNameMadureira
person.familyNameSarmento
person.familyNamePintado
person.givenNamePatrícia
person.givenNameRaquel
person.givenNameBruno
person.givenNameMaria Manuela
person.identifierAAF-7021-2020
person.identifierM-4659-2013
person.identifier152609
person.identifier456608
person.identifier.ciencia-idCB17-644F-65AC
person.identifier.ciencia-id1C14-25F8-CC07
person.identifier.ciencia-idE816-B6B0-06A5
person.identifier.ciencia-id2F13-AAE0-3405
person.identifier.orcid0000-0003-3007-7263
person.identifier.orcid0000-0001-6596-1927
person.identifier.orcid0000-0001-5763-7553
person.identifier.orcid0000-0002-0760-3184
person.identifier.ridM-4659-2013
person.identifier.ridJ-6265-2013
person.identifier.ridF-5696-2013
person.identifier.scopus-author-id37092408800
person.identifier.scopus-author-id8384097900
person.identifier.scopus-author-id57194334236
person.identifier.scopus-author-id7004483898
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublication1f596c0a-945c-480e-9a9e-00f7df7cfc03
relation.isAuthorOfPublicationd33a1d99-423e-4ecd-ae9e-b3e46262b521
relation.isAuthorOfPublication1df2ac4c-2a82-4f3b-9aea-6f4fb9964752
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relation.isAuthorOfPublication.latestForDiscoveryd33a1d99-423e-4ecd-ae9e-b3e46262b521
relation.isProjectOfPublication9681d077-5bd3-4a82-830f-a9a684b77839
relation.isProjectOfPublication.latestForDiscovery9681d077-5bd3-4a82-830f-a9a684b77839

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