Publicação
Analysis of the kinetic patterns of horseradish peroxidase thermal inactivation in sodium phosphate buffer solutions of different ionic strength
| dc.contributor.author | Saraiva, Jorge | |
| dc.contributor.author | Oliveira, Jorge C. | |
| dc.contributor.author | Lemos, Adília | |
| dc.contributor.author | Hendrickx, Marc | |
| dc.date.accessioned | 2011-10-14T15:00:32Z | |
| dc.date.available | 2011-10-14T15:00:32Z | |
| dc.date.issued | 1996 | |
| dc.description.abstract | The thermal inactivation of horseradish peroxidase was studied in sodium phosphate buffer solutions and in pure water at pH 7 in the temperature range of 70–95°C. The sodium phosphate ions concentration affected both the thermostability and the kinetic patterns and had a stabilizing effect. The gradual change observed at low concentrations made a series-type mechanism theoretically more coherent with the experimental observations than the conventionally applied two-fraction model. In water the kinetics is apparently First order at high temperatures, while the results obtained at 25°C support the occurrence of a series-type inactivation mechanism. The pH and enzyme concentration also affect the inactivation proFile, supporting the conclusion that the thermal inactivation is not a monomolecular process with respect to protein concentration. | por |
| dc.identifier.citation | SARAIVA, Jorge [et al] - Analysis of the kinetic patterns of horseradish peroxidase thermal inactivation in sodium phosphate buffer solutions of different ionic strength. International Journal of Food Science and Technology. ISSN 0950-5423. 31:3 (1996) 223-231 | por |
| dc.identifier.doi | 10.1046/j.1365-2621.1996.00342.x | |
| dc.identifier.uri | http://hdl.handle.net/10400.14/6096 | |
| dc.language.iso | eng | por |
| dc.peerreviewed | yes | por |
| dc.publisher | Wiley Blackwell | por |
| dc.subject | Enzyme inactivation | por |
| dc.subject | kinetic models | por |
| dc.title | Analysis of the kinetic patterns of horseradish peroxidase thermal inactivation in sodium phosphate buffer solutions of different ionic strength | por |
| dc.type | journal article | |
| dspace.entity.type | Publication | |
| person.familyName | Oliveira | |
| person.givenName | Jorge | |
| person.identifier.orcid | 0000-0002-0680-6174 | |
| person.identifier.rid | C-2642-2008 | |
| person.identifier.scopus-author-id | 7202452289 | |
| rcaap.rights | restrictedAccess | por |
| rcaap.type | article | por |
| relation.isAuthorOfPublication | 5227bcfa-cb99-407e-a2ef-3f4eb86d4243 | |
| relation.isAuthorOfPublication.latestForDiscovery | 5227bcfa-cb99-407e-a2ef-3f4eb86d4243 |