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Peptide hydrolase system of lactic acid bacteria isolated from Serra da Estrela cheese

2000Journal article Open access
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dc.contributor.authorMacedo, Angela C.
dc.contributor.authorVeira, Marta
dc.contributor.authorPoças, Raquel
dc.contributor.authorMalcata, F. X.
dc.date.accessioned2011-10-19T13:52:39Z
dc.date.available2011-10-19T13:52:39Z
dc.date.issued2000
dc.description.abstractLactobacillus paracasei subsp. paracasei ESB 230, Leuconostoc mesenteroides subsp. mesenteroides ESB 136, Lactococcus lactis subsp. lactis ESB 117 and Enterococcus faecium ESB 50, previously isolated from certi"ed Serra da Estrela cheeses, were tested for their aminopeptidase, dipeptidyl aminopeptidase, endopeptidase, dipeptidase and carboxypeptidase activities. The crude cell-free extracts (CFE) of Lb. paracasei ESB 230 exhibited the highest aminopeptidase activity, followed by CFE of Leuc. mesenteroides ESB 136 and, at last, by CFE of L. lactis ESB 117; the aminopeptidase activity in CFE of Ent. faecium was practically non-existent. The four CFE studied also showed appreciable carboxypeptidase activities, although these were lower than their dipeptidase counterparts; in addition, their dipeptidyl aminopeptidase and endopeptidase activities were lower than their aminopeptidase activities. Dipeptides consisting of hydrophobic amino acid residues (i.e. leucine, methionine and phenylalanine) were more rapidly attacked by all CFE than those with hydrophilic amino acid residues. The peptide hydrolase system of CFE of Lb. paracasei ESB 230 was qualitatively quite similar to, but quantitatively more active than that of CFE of Leuc. mesenteroides ESB 136 (except for the endopeptidase); additionally, the CFE of L. lactis ESB 117 and of Ent. faecium ESB 50 were quite distinct from each other, and from the other two CFE tested.por
dc.identifier.citationMACEDO, Angela C....[et al] - Peptide hydrolase system of lactic acid bacteria isolated from Serra da Estrela cheese. International Dairy Journal. ISSN 0958-6946. Vol. 10 (2000), p. 769-774por
dc.identifier.urihttp://hdl.handle.net/10400.14/6473
dc.language.isoengpor
dc.peerreviewedyespor
dc.publisherElsevierpor
dc.relation.publisherversiondoi:10.1016/S0958-6946(00)00111-4por
dc.subjectDairy foodspor
dc.subjectLactic acid bacteriapor
dc.subjectPeptidase activitypor
dc.titlePeptide hydrolase system of lactic acid bacteria isolated from Serra da Estrela cheesepor
dc.typejournal article
dspace.entity.typePublication
person.familyNameMacedo
person.familyNameMalcata
person.givenNameAngela
person.givenNameFrancisco
person.identifier.ciencia-idE116-EC15-79C5
person.identifier.ciencia-id1B13-38A5-35F5
person.identifier.orcid0000-0002-6854-9855
person.identifier.orcid0000-0003-3073-1659
person.identifier.scopus-author-id7101906752
person.identifier.scopus-author-id7102542478
rcaap.rightsopenAccesspor
rcaap.typearticlepor
relation.isAuthorOfPublication6601534a-2130-4525-bf92-604e4831bb9a
relation.isAuthorOfPublicationa06c00da-0e2f-4434-8ede-4d7b22c0dfe9
relation.isAuthorOfPublication.latestForDiscoverya06c00da-0e2f-4434-8ede-4d7b22c0dfe9

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