Publication
Preparation, characterization and evaluation of guar films impregnated with relaxing peptide loaded into chitosan microparticles
| dc.contributor.author | Batista, Patrícia | |
| dc.contributor.author | Castro, Pedro M. | |
| dc.contributor.author | Madureira, Ana Raquel | |
| dc.contributor.author | Sarmento, Bruno | |
| dc.contributor.author | Pintado, Manuela | |
| dc.date.accessioned | 2021-11-03T15:45:08Z | |
| dc.date.available | 2021-11-03T15:45:08Z | |
| dc.date.issued | 2021-11-01 | |
| dc.description.abstract | Background: Biological molecules such as therapeutic proteins and peptides have provided ample opportunities for further improvements in health and wellbeing. However, such biomacro-molecules face stability challenges regarding premature processing and digestion, whenever any enteric delivery route is considered. Hence, oral guar films entrapping peptide-containing chitosan microparticles have been developed as a new delivery system to carry and protect therapeutic relaxing peptides. Results: The composite films were characterized regarding physicochemical properties using FTIR as well as peptide encapsulation efficiency, cytotoxicity, and peptide permeability through buccal and intestinal cell-based models. The composite films with 0.18% (w/w) of peptide demon-strated ca. 0.63% of peptide loading efficiency into the chitosan microparticles and to be safe for buccal and intestinal epithelial cells up to 24 h of contact. The permeability assays through TR146 cells to mimic buccal mucosa epithelium and Caco-2/HT29-MTX to mimic duodenum conditions showed that the incorporation of peptide chitosan microparticles into guar-gum oral films offered a faster permeability, when compared with the free peptide and peptide-chitosan-microparticles-loaded chitosan. Conclusion: This study suggests that peptide-loaded chitosan microparticles into guar-gum oral films might be a promising carrier for enhancement of the buccal and intestinal absorption of a relaxing peptide. | pt_PT |
| dc.description.version | info:eu-repo/semantics/publishedVersion | pt_PT |
| dc.identifier.doi | 10.3390/app11219849 | pt_PT |
| dc.identifier.eid | 85117747373 | |
| dc.identifier.issn | 2076-3417 | |
| dc.identifier.uri | http://hdl.handle.net/10400.14/35791 | |
| dc.identifier.wos | 000726930000001 | |
| dc.language.iso | eng | pt_PT |
| dc.peerreviewed | yes | pt_PT |
| dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | pt_PT |
| dc.subject | Bioactive peptides | pt_PT |
| dc.subject | Chitosan | pt_PT |
| dc.subject | Oral delivery system | pt_PT |
| dc.subject | Oral films | pt_PT |
| dc.title | Preparation, characterization and evaluation of guar films impregnated with relaxing peptide loaded into chitosan microparticles | pt_PT |
| dc.type | journal article | |
| dspace.entity.type | Publication | |
| oaire.citation.issue | 21 | pt_PT |
| oaire.citation.title | Applied Sciences (Switzerland) | pt_PT |
| oaire.citation.volume | 11 | pt_PT |
| rcaap.rights | openAccess | pt_PT |
| rcaap.type | article | pt_PT |
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