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Influence of sulphate on the composition and antibacterial and antiviral properties of the exopolysaccharide from Porphyridium cruentum

2014Journal article Restricted access
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dc.contributor.authorRaposo, Maria Filomena de Jesus
dc.contributor.authorMorais, Alcina Maria Miranda Bernardo de
dc.contributor.authorMorais, Rui Manuel Santos Costa de
dc.date.accessioned2014-05-08T13:42:18Z
dc.date.available2014-05-08T13:42:18Z
dc.date.issued2014
dc.description.abstractAims: The influence of two culture media and three different concentrations of sulphate in the medium on the growth of two strains of Porphyridium cruentum and on the production, composition and viscoelastic characteristics, and antimicrobial properties of the sulphated exopolysaccharide (EPS) were studied. Mainmethods: A Bohlin C50 rheometerwas used to evaluate the viscosity and elasticity of the EPS solutions. HSV virus, types 1 and 2, Vaccinia virus and Vesicular stomatitis virus were used along with two Gram-negative (Escherichia coli and Salmonella enteritidis) and one Gram-positive (Staphylococcus aureus) bacteria, for testing the antimicrobial activity of EPS. Key-findings: The growth ofmicroalgae was higher in NTIP medium and the production of EPS was enhanced by sulphate 21 mM. The protein content of the EPS was enhanced by the addition of sulphate 52mMand 104mM; this concentration also induced an increase in sulphate content of the EPS. However, neither the contents of EPS in carbohydrates and uronic acidswere affected by the culturemediumsupplementation in sulphate. In general, the EPS from the Spanish strain presented a higher antiviral activity than the EPS from the Israeli strain. All EPS extracts revealed a strong activity against V. stomatitis virus, higher than the activity of all chemical compounds tested. The EPS from the Israeli strain also presented antibacterial activity against S. enteritidis. Significance: Enrichment of the culture mediumwith sulphate improved protein and sulphate content of EPS. EPS extracts presented a relevant activity against V. stomatitis virus and S. enteritidis bacterium.por
dc.description.versioninfo:eu-repo/semantics/publishedVersion
dc.identifier.citationRAPOSO, Maria Filomena de Jesus; MORAIS, A.M.M.B.; MORAIS, R.M.S.C. - Influence of sulphate on the composition and antibacterial and antiviral properties of the exopolysaccharide from Porphyridium cruentum. Life Sciences. ISSN 0024-3205. Vol. 101, n.º 1-2 (2014), p. 56–63por
dc.identifier.doi10.1016/j.lfs.2014.02.013
dc.identifier.urihttp://hdl.handle.net/10400.14/14315
dc.identifier.wosWOS:000334983500008
dc.language.isoengpor
dc.peerreviewedyespor
dc.publisherElsevier
dc.relationADI/2006/M2.3/0032
dc.subjectRed microalgapor
dc.subjectPorphyridium cruentumpor
dc.subjectSulphated exopolysaccharidepor
dc.subjectCulture media modificationspor
dc.subjectAntiviral activitypor
dc.subjectAntibacterial activitypor
dc.titleInfluence of sulphate on the composition and antibacterial and antiviral properties of the exopolysaccharide from Porphyridium cruentumpor
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage63
oaire.citation.startPage56
oaire.citation.volume101
person.familyNamede Jesus Raposo
person.familyNameBernardo
person.familyNameMorais
person.givenNameMaria Filomena
person.givenNameAlcina M.M.
person.givenNameRui M. S. C.
person.identifierM-5215-2013
person.identifier143926
person.identifier.ciencia-id4F1A-88A7-FB5C
person.identifier.ciencia-idEB10-A13C-2748
person.identifier.ciencia-id541B-04AE-0686
person.identifier.orcid0000-0002-2411-0410
person.identifier.orcid0000-0002-1059-9649
person.identifier.orcid0000-0001-5138-6941
person.identifier.ridM-5215-2013
person.identifier.ridM-5065-2013
person.identifier.scopus-author-id7005684061
person.identifier.scopus-author-id7006325245
person.identifier.scopus-author-id7005146997
rcaap.rightsrestrictedAccesspor
rcaap.typearticlepor
relation.isAuthorOfPublication3a13dab3-c68c-4db4-a3a1-6ba8507f92b5
relation.isAuthorOfPublication7d4bb84a-d049-417f-9d54-8b48219af15f
relation.isAuthorOfPublicationfa2b729c-8159-4a98-9bfe-0fa1e98aa9fb
relation.isAuthorOfPublication.latestForDiscoveryfa2b729c-8159-4a98-9bfe-0fa1e98aa9fb

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