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Proteolysis of ovine and caprine Caseins in solution by enzymatic extracts from flowers of Cynara cardunculuso

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dc.contributor.authorSousa, M. José
dc.contributor.authorMalcata, F. Xavier
dc.date.accessioned2011-10-21T12:55:36Z
dc.date.available2011-10-21T12:55:36Z
dc.date.issued1998
dc.description.abstractPrimary proteolysis of ovine and caprine Na-caseinate at 30°C in phosphate buffer at pH 6.5 or 5.5 in the absence of NaCl and at pH 5.2 with 5% (w/v) NaCl by cardosins in aqueous extracts of Cynara cardunculus flowers was investigated using urea-polyacrylamide gel electrophoresis and reversed-phase high performance liquid chromatography. Caprine caseinate underwent more extensive degradation than ovine caseinate under the same conditions (pH 6.5 and pH 5.5); proteolysis of b- and as-caseins in ovine and, to a lesser extent, in caprine caseinates was reduced in the presence of 5% (w/v) NaCl. Peptide profiles of the pH 4.6-soluble extract had different patterns throughout ripening arising from the different specificity of cardosins toward ovine and caprine Na-caseinates. The major cleavage sites in ovine (caprine) caseinate were Phe105-Met106 (Lys116-Thr117) for k-casein, Leu127-Thr128 and Leu190-Tyr191 (Glu100-Thr101, Leu127-Thr128, Leu136-Pro137 and Leu190- Tyr191) for b-casein, Phe23-Val24 (Phe23-Val24, Trp164-Tyr165 and Tyr173-Thr174) for as1-casein and Phe88-Tyr89 (Ser9-Ser10, Phe88-Tyr89 and Tyr179-Leu180) for as2-casein.por
dc.identifier.citationSOUSA, M. José; MALCATA, F. Xavier - Proteolysis of Ovine and Caprine Caseins in Solution by Enzymatic Extracts from Flowers of Cynara cardunculus. Enzyme and Microbial Technology. ISSN 0141-0229. Vol. 22 (1998), p. 305–314por
dc.identifier.urihttp://hdl.handle.net/10400.14/6660
dc.language.isoengpor
dc.peerreviewedyespor
dc.publisherElsevierpor
dc.relation.publisherversiondoi:10.1016/S0141-0229(97)00173-7por
dc.subjectPlant rennetpor
dc.subjectCheese ripeningpor
dc.subjectProteasespor
dc.subjectEnzyme specificitypor
dc.titleProteolysis of ovine and caprine Caseins in solution by enzymatic extracts from flowers of Cynara cardunculusopor
dc.typejournal article
dspace.entity.typePublication
person.familyNameMalcata
person.givenNameFrancisco
person.identifier.ciencia-id1B13-38A5-35F5
person.identifier.orcid0000-0003-3073-1659
person.identifier.scopus-author-id7102542478
rcaap.rightsopenAccesspor
rcaap.typearticlepor
relation.isAuthorOfPublicationa06c00da-0e2f-4434-8ede-4d7b22c0dfe9
relation.isAuthorOfPublication.latestForDiscoverya06c00da-0e2f-4434-8ede-4d7b22c0dfe9

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