Novo título: Toxicometabolomics of amphetaminic derivatives in in vitro and in vivo experimental models Antigo: Evaluation of toxicological effects mediated by synthetic cathinones through the application of GC-MS metabolomic studies using in vitro/in vivo models

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Sensory attributes and volatile composition of a dry white wine under different packing configurations

Publication . Moreira, N.; Lopes, P.; Ferreira, H.; Cabral, M.; Pinho, P. Guedes de

The aim of this work was to study the effect of different configurations of packaging on the volatile composition and sensory properties of a white wine. Certain oenological parameters were also evaluated. Bag-in-box (BIB) and glass bottles sealed with two different cork stoppers, natural and Neutrocork (technical), were used in the experiments. Analysis were carried out before packaging and after 3, 6 and 12 months of storage. Results showed that wines packaged in BIB presented higher levels of brown color than wines in bottles sealed with corks. In all packaging configurations, the content of free SO2 decreased with storage time; however, BIB wines showed a lower content of free SO2 than bottle wines during 12 months. Moreover, wines under BIB presented a significant lower amount of 2-phenylethanol, 2-phenylethyl acetate, isoamyl acetate, ethyl butanoate, ethyl hexanoate, ethyl octanoate, linalool and b-damascenone than bottled wines.

2018Journal article

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Development and optimization of a HS-SPME-GC-MS methodology to quantify volatile carbonyl compounds in Port wines

Publication . Moreira, Nathalie; Araújo, Ana Margarida; Rogerson, Frank; Vasconcelos, Isabel; Freitas, Victor de; Pinho, Paula Guedes de

A method based on headspace solid-phase microextraction (HS-SPME) coupled to gas chromatography-triple quadrupole/mass spectrometry detection (GC-TQ/MS) with a prior derivatization step with O-(2,3,4,5,6-pentafluorobenzyl)hydroxylamine hydrochloride (PFBHA) was developed to quantify carbonyl compounds in different categories of Port wines. Optimal extraction conditions were obtained incubating 2 ml of wine with 2.3 g/l of PFBHA for 10 min and extracted during 20 min at 32 °C. The method was validated for 38 carbonyl compounds (alkanals, alkenals, Strecker aldehydes, dialdehydes, ketones and furan aldehydes) with regard to linearity, repeatability, inter and intra-day precision and accuracy, showing that the method is suitable for the determination of carbonyl compounds in wines. Tawny wines with ‘indication of age’ (10–40 years old) presented the highest levels of some carbonyl compounds, such as propanal, pentanal, hexanal, Strecker aldehydes, diacetyl, methyl glyoxal, 3-pentanone and 2-furfural, whereas Ruby wines were characterized by the highest amounts of some unidentified compounds.

2019Journal article

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