Percorrer por autor "Socodato, Renato"
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- CLA and CLNA ameliorate neuroinflammation and cellular oxidation related with western dietsPublication . Salsinha, Ana Sofia; Socodato, Renato; Rodrigues, Artur; Rodríguez-Alcalá, Luis Miguel; Relvas, João B.; Pintado, Manuela E.High-fat diet has been associated with a chronic-low grade inflammation in both adipose tissue and central nervous system. Fatty acids are known to cross the blood-brain barrier and reach the central nervous system where they can accumulate. Microglia express a wide range of lipid-sensitive receptors, potentially triggering inflammatory responses. Since fatty acids can exert pro and anti-inflammatory effects in the hypothalamus, in this work, through live cell imaging and FRET technology, we assessed the potential role of omega-3 fatty acids and CFAs in modulating microglia inflammation triggered by obesogenic nutrients. First, the combined action of fructose and saturated fatty acid palmitic acid (to mimic western pattern diet), induced NFkB pathway activation and oxidative stress, by reactive oxygen species production, in HMC3 human microglia. Such results suggest that western pattern diet may induce microglia inflammatory processes in the central nervous system, ultimately resulting in neuroinflammation. On the other hand, exposure of HMC3 cells to polyunsaturated fatty acids (omega-3 – EPA and DHA- and CLA and CLNA isomers) showed a preventive potential, since they were able to abolish the palmitic acid+fructose induced-NFkB pathway activation. Moreover, omega-3 and CLA also showed antioxidant potential by inhibition of reactive oxygen species production. Although the mechanisms of action have not been fully described yet, GPR120/FFA4 is known to bind some omega-3 fatty acids. By using chemical agonists and antagonists of GPR120/FFA4 it was demonstrated that while omega-3, CLA and CLNA effect on NFkB pathway inhibition is mediated by this receptor, the antioxidant ability of omega-3 and CLA occurs through different signaling mechanisms. It was suggested, for the first time, that CLA and CLNA have a similar action to omega-3 on microglia, probably via GPR120 activation and modulation of NFkB-associated inflammatory pathways.
- CLA and CLNA ameliorate neuroinflammation and cellular oxidation related with western dietsPublication . Salsinha, Ana Sofia; Socodato, Renato; Rodrigues, Artur; Rodríguez-Alcalá, Luis Miguel; Relvas, João B.; Pintado, Manuela E.
- Structure and function of a novel antioxidant peptide from the skin of tropical frogsPublication . Barbosa, Eder Alves; Oliveira, Ana; Plácido, Alexandra; Socodato, Renato; Portugal, Camila C.; Mafud, Ana Carolina; Leite, José Roberto S.A.; Pintado, M. E.The amphibian skin plays an important role protecting the organism from external harmful factors such as microorganisms or UV radiation. Based on biorational strategies, many studies have investigated the cutaneous secretion of anurans as a source of bioactive molecules. By a peptidomic approach, a novel antioxidant peptide (AOP) with in vitro free radical scavenging ability was isolated from Physalaemus nattereri. The AOP, named antioxidin-I, has a molecular weight [M+H]+ = 1543.69 Da and a TWYFITPYIPDK primary amino acid sequence. The gene encoding the antioxidin-I precursor was expressed in the skin tissue of three other Tropical frog species: Phyllomedusa tarsius, P. distincta and Pithecopus rohdei. cDNA sequencing revealed highly homologous regions (signal peptide and acidic region). Mature antioxidin-I has a novel primary sequence with low similarity compared with previously described amphibian's AOPs. Antioxidin-I adopts a random structure even at high concentrations of hydrophobic solvent, it has poor antimicrobial activity and poor performance in free radical scavenging assays in vitro, with the exception of the ORAC assay. However, antioxidin-I presented a low cytotoxicity and suppressed menadione-induced redox imbalance when tested with fibroblast in culture. In addition, it had the capacity to substantially attenuate the hypoxia-induced production of reactive oxygen species when tested in hypoxia exposed living microglial cells, suggesting a potential neuroprotective role for this peptide.
- Study of inflammation mediated by lipids in a microglia cell model to deepen into brain dysregulation by obesityPublication . Salsinha, A S; Rodrigues, A; Socodato, Renato; Relvas, J B; Rodríguez-Alcalá, L M; Pintado, M E
- The pro- and anti-inflammatory activity of fatty acidsPublication . Salsinha, Ana Sofia; Socodato, Renato; Relvas, João B.; Pintado, ManuelaInflammation is crucial to maintain homeostasis in the body. The contribution of fatty acids to the inflammatory process is exerted through a variety of mechanisms leading to cell surface modifications, activation of intracellular receptors that control inflammatory signaling processes, and changes in gene expression patterns. While long-chain saturated fatty acids induce NFkB pathway activation through TLR-4 binding, unsaturated fatty acids, such as monounsaturated, polyunsaturated, and conjugated fatty acids’ antiinflammatory ability is mediated through PPARs or GPR120. Moreover, these unsaturated fatty acids, especially omega-3 fatty acids, have immunomodulatory and cytoprotective potential, which is highly relevant for diseases with a neuroinflammatory component, such as obesity, Alzheimer’s disease, multiple sclerosis, and schizophrenia.