Browsing by Author "Rodrigues, Daniela"
Now showing 1 - 5 of 5
Results Per Page
Sort Options
- Antimicrobial potential of formulations, incorporating spent yeast derived from synthetic biotechnology, against Pseudomonas spp.Publication . Deuchande, Teresa; Fundo, Joana; Rodrigues, Daniela; Souza, Carla; Pintado, Manuela; Amaro, Ana L.Yeasts are currently used as cell factories for the sustainable production of high value biomolecules for applications in the pharmaceutical and cosmetic sectors. However, the production of such molecules through fermentation, conducted in bioreactors and making use of genetically engineered yeast strains, generates a number of waste-streams, with spent yeast as the second main by-product of fermentation processes, representing more than 20% of the total waste produced. Since this by-product is rich in several molecules including proteins, vitamins and several minerals, being also a natural source of glucans and mannoproteins with known bioactivities, it is of interest to develop valorization strategies for these residues. In this work, spent yeast was autolysed and a set of formulations, incorporating the resulting supernatant and pellet at different concentrations, were tested as potential antimicrobial solutions to prevent diseases in plants and fungi caused by Pseudomonas spp. The formulations were initially tested against P. aeruginosa and then against strains affecting cultivated mushrooms (Agaricus bisporus), P. tolaasi, and P. agarici; and a number of P. syringae strains responsible for plant diseases including the pathovars actinidifoliorium, tomato, pisi, syringae and atrofaciens. It has been recently shown that spent yeast hydrolysates possess antimicrobial activity against Salmonella enterica, Aeromonas salmonicida, Bacillus cereus and Bacillus subtilis (Martin et al. 2021), suggesting that spent yeast extracts may have potential antimicrobial effect against a range of microorganisms. To date, there are no studies showing the potential of spent yeast extracts against Pseudomonas strains. The results showed that the supernatant of the autolyzed yeast at 0.1% inhibited the growth of P. aeruginosa by about 20% and P. tolaasii by 10% but increased the growth of P. agarici by up to 34%. The combination of supernatant with lactose also slightly increased P. tolaasii growth inhibition (12%) but it reduced the inhibitory effect of supernatant against P. aeruginosa. With regard to plant pathogens, the formulations containing 0.1% and 0.3% of pellet inhibited by 25% the growth P. syringae pv. actinidifoliorium, the bacterium responsible for kiwi canker, but for all the other pathovars, the inclusion of spent yeast extracts in the formulation induced bacterial growth. Among all the tested formulations, the ones including supernatant and pellet at low concentrations were the most promising leading to slight growth inhibition of some environmental Pseudomonas spp.
- Characterization of spent yeast streams from precision fermentations driven by genetically engineered yeasts: valorization potential for a circular bioeconomyPublication . Deuchande, Teresa; Fundo, Joana F.; Rodrigues, Daniela; Pintado, Manuela E.; Amaro, Ana L.Currently, valuable biomolecules are sustainably produced through fermentation using genetically modified microorganisms. However, this industry also generates several waste streams, such as spent yeast (SY). To close the sustainability loop in bioproduction, it is essential to define valorization strategies for such waste streams. Hence, this study aimed to investigate the compositional differences among these novel SYs, intending to pave the way for devising highly effective and beneficial valorization strategies for these streams. In this work, SYs derived from the production of beer (SBY) and six biomolecules [β-farnesene (Fene), rebaudioside M (Reb M), manool (MN), patchouli (PAT), sandalwood (SDW), and sclareol (SCL)] through fermentations driven by engineered yeasts were characterized. The results showed that SY from Fene, MN, and PAT production have similar concentrations of protein (35.9–44% dw), minerals, and amino acids, being compatible with bulking and use as nitrogen sources. Those derived from Reb M production showed significantly higher levels of lipids (21 ± 2.6% dw) compared to all the other SYs (1.7–10.8% dw), whereas the ones derived from SCL production showed significantly higher levels of inorganic matter (45%) compared with those found in the other SYs (1.2–11.3% dw). The results showed that assessing the extent of compositional variation among different SYs derived from precision fermentations represents a primary step toward developing efficient valorization or biorefining strategies.
- Sedación em cuidados paliativos: Una revisión integradoraPublication . Coelho, Patrícia; Afonso, Diogo; Araújo, Helena; Barbas, Ana; Rodrigues, Daniela; Silva, Cristiana; Silva, Vânia; Vilhena, Alícia; Mello, Ramón Andrade de
- The impact of metabolic engineering of yeast, designed to produce valuable biomolecules through fermentation, on resulting spent yeast compositionPublication . Deuchande, Teresa; Fundo, Joana; Rodrigues, Daniela; Amaro, Ana
- Valorization of biotechnology derived spent yeast as potential ruminant feed additivePublication . Souza, Carla Giselly de; Rodrigues, Daniela; Deuchande, Teresa; Fundo, Joana; Pintado, Manuela E.; Amaro, Ana L.The production of high quantities of spent yeast is a transverse problem to most industries based on fermentation processes. Traditional fermentation industries, such as breweries, and the ones that use engineered yeast to produce high commercial interest molecules, have been looking for strategies to minimize this problem. Spent yeast represents a valuable nutrient source due to its abundant levels of several high value bioactive compounds. Through an optimized autolysis process, high levels of these bioactive compounds, such as β-glucans, mannans, peptides, and other nutrients can be more easily available, targeting several commercial applications. Some of these compounds have prebiotic applicability in animal feed industries. The benefits of supplementing yeast in diets of ruminants trusts on the increase of cellulolytic bacteria in the rumen, increasing the energy extracted from the diets, and consequently the animal’s performance (Bortoluzzi et al., 2018). Yeast derivatives have shown the potential to modulate ruminal fermentation by decreasing the starch degradation rate or by stimulating the growth of lactate-utilizers in the rumen. As the effect of it may be increased by low pH or by high-concentrate diets, supplementing the ruminant's diet with autolyzed yeast may help to counteract the negative effects of high-concentrate diets (Kröger et al., 2017; Humer et al., 2018; Neubauer et al., 2018). This research aimed to characterize a synthetic biotechnology fermentation derived spent yeast as potential additive to ruminant feed. Autolyzed spent yeast was spray dried and characterized in terms of composition and prebiotic potential. The challenge in the utilization of traditional fermentation derived spent yeast, for ruminant consumption, is to guarantee the level of protein and essential amino acids, their ratio and stability along storage. Results demonstrate that synthetic biology associated fermentation derived spent yeast presented both nutritional value and prebiotic activity showing high potential to be incorporated as an additive for ruminants feed, with an interesting amino acids profile.