Percorrer por autor "Reis, Mariana S."
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- Detergent-free supercritical CO2–assisted protocol for the production of sustainable and highly preserved decellularized porcine meniscus for orthopedic applicationsPublication . Ho, Chou I.; Rodrigues, Francisco A. P.; Reis, Mariana S.; Ribeiro, Viviana P.; Oliveira, Ana Leite; Costa, João B.
- Detergent-free supercritical CO2–assisted protocol for the production of sustainable and highly preserved decellularized porcine meniscus for orthopedic applicationsPublication . Ho, Chou I.; Rodrigues, Francisco A. P.; Reis, Mariana S.; Ribeiro, Viviana P.; Oliveira, Ana Leite; Costa, João B.Introduction & Objectives Meniscal injuries occur approximately 66 to 70 per 100,000 individuals annually (Fig. 1), potentially leading to the development of osteoarthritis (OA) or other degenerative cartilage disease in 10 to 20 years. One of the conventional treatments is meniscal allograft transplantation. However, its limitations constrain its comprehensive application in the healthcare system.
- Detergent-free supercritical CO2–assisted protocol for the production of sustainable and highly preserved decellularized porcine meniscus for orthopedic applicationsPublication . Ho, Chou I.; Rodrigues, Francisco A. P.; Reis, Mariana S.; Ribeiro, Viviana P.; Oliveira, Ana L.; Costa, João B.
- Development of a decellularized extracelular matrix from porcine aorta for heart valve applications in the Ross procedurePublication . Reis, Mariana S.; Rosadas, Marta; Ho, Chou I.; Costa, João; Vervenne, Thibault; Oliveira, Ana L.; Ribeiro, Viviana P.; Mignon, ArnCardiovascular diseases are the leading cause of adult mortality worldwide, according to the WHO [1]. An important surgical approach for treating diseased aortic valves is the Ross procedure, in which the affected aortic valve is replaced with an autograft from the patient’s own pulmonary valve. The main concern about this procedure is linked to wall dilatation, which can lead to valve leakage and reoperation. Dilatation occurs due to the fivefold increase in blood pressure when transitioning from pulmonary to aortic conditions. Current solutions rely on permanet and stiff synthetic materials to provide structural support, however, these lack biological functionality. Our approach aim to incorporate a decellularized extracellular matrix (dECM) in a semi-permanent textile wrapped around the autograft promoting benign biological adaptation. The decellularization process by removing cellular components, reduces the risk of inflammatory responses and immune rejection. Moreover, essential ECM components that regulate cellular behavior are mantained, which is crucial for effective decellularization outcomes [2]. This study is focused in the development and optimization of an efficient decellularization protocol for obtaining dECM from porcine aorta with mild effects on ECM components preservation. Decellularization of the porcine aortic tissue was performed using a detergent and enzymatic-based protocol combined with supercritical CO₂ (scCO₂). Additional steps of sonication, agitation, washing and freeze-thaw were performed to enhance decellularization efficiency. Graphic A shows a significant decrease in the DNA content after optimized decellularization processing, indicating the removal of approximatly 85% of the DNA from the native tissue, and suggest an effective reduction of potential immunogenic components. Further characterization of ECM components (i.e. glycosaminoglycans, total collagen and elastin) is required to evaluate its preservation and the biological potencial of the dECM when integrated in the Ross processure. To do so, aorta dECM powder will be combined with polycaprolactone and elastin and processed through electrospinning used to create a tubular device to enhance bio-mechanocompatibility, further improving current external supports for the Ross procedure and enhancing its general clinical outcomes. Since this support is biodegradable, it allows the new valve to take over the required strength. The dECM specifically offers essential biochemical cues to promote tissue integration and enable long-term functional repair after the Ross procedure.
- Development of a decellularized extracelular matrix from porcine aorta for heart valve applications in the Ross procedurePublication . Reis, Mariana S.; Rosadas, Marta; Ho, Chou I.; Sousa, Teresa; Pazmino, Carlos; Costa, João; Vervenne, Thibault; Oliveira, Ana L.; Ribeiro, Viviana P.; Mignon, ArnCardiovascular diseases are the leading cause of adult mortality worldwide, according to the World Health Organization [1]. An important surgical approach for treating diseased aortic valves is the Ross procedure, in whic the affected aortic valve is replaced with an autograft from the patient’s own pulmonary valve.
- Going green and mimetic: new ECM-based hydrogel for meniscus regeneration using supercritical CO2-assisted decellularizationPublication . Ho, Chou I.; Rodrigues, Francisco A. P.; Reis, Mariana S.; Sousa, Clara; Ribeiro, Viviana P.; Oliveira, Ana L.; Costa, João B.Meniscal injuries and its subsequent progression to osteoarthritis represent a major clinical challenge, as current treatments often fail to achieve effective and complete functional restoration. An alternative regenerative approach is tissue engineering using decellularized extracellular matrix (dECM), which aims to restore meniscal structure and function. Porcine meniscus, an abundant byproduct of meat production, offers a readily available source for this purpose. Unlike conventional decellularization methods that are harsh, time-consuming, and detrimental to extracellular matrix (ECM) integrity, a detergent-free decellularization protocol utilizing supercritical carbon dioxide (scCO2) fluid was developed. This sustainable and minimally-invasive process was employed in cyclic pressurization-depressurization dynamics and scCO2 fluid was applied for sterilization by incorporating oxidative additives. Quantitative and qualitative analyses using quantification extraction kits and Fourier transform infrared (FTIR) spectroscopic analysis, presented as principal component analysis (PCA) and loading plots through a Machine Learning Toolbox, demonstrated substantial preservation of key ECM components. Sterilization efficiency was also confirmed using turbidity tests. dECM residual DNA content results fell within the range of general benchmark of 50ng/mg of dry tissue and the endotoxin levels, recently recognized as critical cause of host responds and regenerative outcomes [1], were below 20 endotoxin units (EU). The dECM showed cytocompatibility in vitro and an injectable hydrogel was successfully developed. The dECM hydrogel presented adhesive properties and was optimized to be used as a bioink for 3D bioprinting approaches.