Browsing by Author "Oumeima, Boufercha"
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- Biodegradation of 2,4 DCP herbicide by streptomyces collinus isolated from wastewater treatment plant in eastern AlgeriaPublication . Oumeima, Boufercha; Allaoueddine, Boudemagh; Moreira, Irina Sousa; Castro, Paula Maria LimaWastewater treatment plants are the place where most pollutants are transported. 2, 4 DCP is an herbicide widely used in agriculture. This carcinogenic pollutant is very dangerous because it can reach surface waters through runoff and deep waters widely used by humans and animals. Water treatment plants are a reservoir of multiple and varied microorganisms, able to eliminate the toxic effect of many pollutants. The actinobacteria by their impressive metabolic abilities, are among the most appreciated microbial agents in the bioremediation of these hydric sites. In order to evaluate the functionality of the Ibn Ziad station in Constantine, we tested some physicochemical characteristics and the biodiversity of actinobacteria able to tolerate and degrade 2, 4 DCP. Sampling was carried out on raw wastewater, treated water and aeration tank water. The parameters studied were temperature, pH, conductivity, salinity, BOD5, DOC and suspended matters (MES). Actinobacteria were isolated on four selective media, namely AF, modified Czapeck dox, ISP4, Olson. The determination of herbicide biodegradation capacity by these bacteria was tested first on a minimum solid medium supplemented with 50 mg/L of 2, 4-DCP as a single carbon and energy source. Isolates that grew on this medium were cultured in liquid medium in the presence of 50 mg/L of the same pollutant. The degradation kinetics were monitored by HPLC. The best performing isolate was identified by phenotypic and molecular methods. The results show slightly alkaline pH, ambient temperatures. The DOC/BOD5 ratio is less than three, which indicates a slightly biodegradable effluent. While the MES concentration is around 256.7 mg/L. This station shows an important biodiversity of actinobacteria, with 25 isolates, among which 18 are able to live in the presence of 2, 4-DCP. The study of the kinetics of growth and degradation shows a good performance of an isolate, with a degradation rate of 45% after one month of incubation. The polyphasic identification of this bacterium, allows to assign it to the species Streptomyces collinus strain NBRC 12759 16S. These results show that the waters of thisstation are rich in actinobacteria able to degrade the herbicide 2, 4-DCP. These bacteria can be used in the bioremediation of water ecosystems polluted by this phytosanitary product
- Biodegradation of 2,4 DCP herbicide by streptomyces collinus isolated from wastewater treatment plant in eastern AlgeriaPublication . Oumeima, Boufercha; Allaoueddine, Boudemagh; Moreira, Irina Sousa; Castro, Paula Maria Lima
- Isolation and identification of actinomycetes degrading the fungicid Allial from the activated sludge of the wastewater treatment plant Ibn Ziad (Constantine city)Publication . Oumeima, Boufercha; Moreira, Irina Sousa; Castro, Paula Maria Lima; Allaoueddine, BoudemaghThe old methods of agriculture were based on the use of intensive manual labor. The twentieth century saw the birth of a revolution in agricultural practices, which relied mainly on plant protection techniques. These new perspectives have paved the way for an agriculture dominated by pesticides. The use of these molecules has improved yields and crop diversity to meet the nutritional needs of the growing world population. However, in recent years, other health and environmental problems have arisen due to uncontrolled overuse. Allial (fosetyl aluminum) is a systemic fungicide of the phosphonate class. It is not very persistent in soils and water and is recalcitrant to hydrolysis at environmental pH. This fungicide is also very soluble in water and can therefore reach surface waters by leaching from agricultural soils. Photolysis of this xenobiotic is not possible. In order to remove this fungicide from wastewater, biological treatment methods are necessary. Activated sludge microorganisms can play an important role in its biodegradation. In this study, were isolated actinomycetes from activated sludge of the wastewater treatment plant of Ibn Ziad. These bacteria, known by their extraordinary metabolic diversities, were tested for their aptitudes to degrade this toxic pesticide. Minimum salt medium supplemented with different concentrations up to 200 mg/L of fosetyl aluminum as the only carbon source. The cultures were incubated during 5 days at a temperature of 30° C. According to the results obtained, five isolates of actinobacteria showed a significant potential for the biodegradation of this fungicide. The identification of these isolates by analysis of the 16S rDNA gene allowed to assign them to the Streptomyces genus.