Browsing by Author "Maouni, Abdelfettah"
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- Phytochemical analysis, antioxidant, antifungal activity, and genotoxicity of essential oil and solvent extracts of tetraclinis articulata leaves from northern MoroccoPublication . Benoutman, Amina; Erbiai, El Hadi; Ettakifi, Hajar; Legssyer, Mounir; Handaq, Nadia; Makrane, Hanane; Saidi, Rabah; Lamrani, Zouhaire; Pintado, Manuela; Pinto, Eugénia; Silva, Joaquim C. G. Esteves da; Maouni, AbdelfettahTetraclinis articulata is a medicinal plant commonly known in Morocco as” Araar Tree “. Various parts of T. articulata are traditionally used for various therapeutic purposes. In this context, the present study aims to evaluate the antioxidant, antifungal, and genotoxicity of methanolic (TaLME), acetonic (TaLAE) extracts, and essential oil (TaLEO) from Tetraclinis articulata leaves growing in northern Morocco. This was carried out through chemical analysis, bioactive substance determination, and essential oil and extract testing. The bioactive substances determined by the spectrophotometric method showed a higher content of phenols and flavonoids in TaLME. The Liquid chromatography-mass spectrometry (LC-MS) analysis of phenolic compounds revealed protocatechuic acid as predominant in TaLME, followed by paraben acid and syringic acid, and in TaLAE, p-hydroxybenzoic was the principal compound, followed by protocatechuic acid and vanillic acid. The gas chromatography-mass spectrometry (GC-MS) analysis of TaLEO (yield of 0.77 %) revealed the presence of 22 compounds (95.80 %) with main components (%) α-pinene (29.62), bornyl acetate (21.45), and camphor (16.80). Biological activities were evaluated: antioxidant efficiency using four different methods (DPPH, ABTS, FRAP, ORAC), deoxyribonucleic acid (DNA) protection, genotoxicity test, and antifungal activity against five pathogenic fungi (Aspergillus fumigatus, Candida albicans, Epidermophyton floccosum, Trichophyton rubrum, and Microsporum canis). According to the results, the extracts and EO had significant antioxidant and antifungal activity against the dermatophytes. The genotoxicity test exhibited that the T. articulata extracts and EO were bereft of any mutagenic activity. Overall, this study demonstrates that Tetraclinis articulata plant essential oil and extracts can represent an intriguing potential source of natural compounds that can be helpful for future investigations.
- Phytochemical composition, antioxidant and antifungal activity of thymus capitatus, a medicinal plant collected from Northern MoroccoPublication . Benoutman, Amina; Erbiai, El Hadi; Edderdaki, Fatima Zahra; Cherif, El Khalil; Saidi, Rabah; Lamrani, Zouhaire; Pintado, Manuela; Pinto, Eugénia; Silva, Joaquim C. G. Esteves da; Maouni, AbdelfettahThymus capitatus is a Mediterranean endemic plant commonly known as “Zaïtra” in northern Morocco. As T. capitatus is widely used in traditional medicine and food, this present work aims to investigate the chemical compositions and biological activities of the T. capitatus leaves essential oil (TcLEO), acetonic (TcLAE), and methanolic extract (TcLME). The spectrophotometric determination demonstrated that T. capitatus is a natural source rich in phenolic contents (TPC) and flavonoid contents (TFC) and that TcLME revealed the highest TPC and TFC than TcLAE and TcLEO. The LC-MS analysis of phenolic compounds showed that paraben acid was predominant in both TcLME and TcLAE, followed by cinnamic acid and p-hydroxybenzoic acid. GC-MS analysis of the TcLEO revealed the presence of a total of 10 compounds, which were predominated by carvacrol. The antioxidant activity by ORAC was observed to be significantly higher in TcLEO and TcLAE than in TcLME. All samples used to assess DNA degradation effectively prevented DNA oxidation and, at the same time, had a prooxidant effect. The genotoxicity test showed that the T. capitatus were devoid of any mutagenic activity. Concerning antifungal activity, all samples were able to inhibit the growth of all microorganisms tested at low concentrations. TcLAE showed higher activity than TcLME, and in general, dermatophytes were more susceptible, being Microsporum canis the most sensitive one. Overall, the results obtained from this study confirm the wide uses of T. capitatus. Furthermore, the finding results suggest that the T. capitatus essential oil and extracts can be highly useful for pharmaceutical industries.