Browsing by Author "Gorecki, Adrian"
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- Development and validation of novel PCR primers for identification of plasmid-mediated colistin resistance (mcr) genes in various environmental settingsPublication . Gorecki, Adrian; Musialowski, Marcin; Wolacewicz, Mikolaj; Decewicz, Przemyslaw; Ferreira, Catarina; Vejmelkova, Dana; Grzesiuk, Malgorzata; Manaia, Célia M.; Bartacek, Jan; Dziewit, LukaszAntibiotic resistance is considered one of the biggest threats to public health and has become a major concern for governments and international organizations. Combating this problem starts with improving global surveillance of antibiotic resistance genes (ARGs) and applying standardized protocols, both in a clinical and environmental context, in agreement with the One Health approach. Exceptional efforts should be directed to controlling ARGs conferring resistance to Critically Important Antimicrobials (CIA). In this study, a systematic literature review to synthesize data on the identification of mcr genes using a PCR technique was performed. Additionally, a novel set of PCR primers for mcr-1 – mcr-9 genes detection was proposed. The developed primers were in silico and experimentally validated by comparison with mcr-specific PCR primers reported in the literature. This validation, besides being a proof-of-concept for primers’ usefulness, provided insight into the distribution of mcr genes in municipal wastewater, clay and river sediments, glacier moraine, manure, seagulls and auks feces and daphnids from four countries. This analysis proved that commonly used primers may deliver false results, and some mcr genes may be overlooked in tested samples. Newly-developed PCR primers turned out to be relevant for the screening of mcr genes in various environments.
- Monitoring antibiotic resistance genes in wastewater environments: the challenges of filling a gap in the One-Health cyclePublication . Miłobedzka, Aleksandra; Ferreira, Catarina; Vaz-Moreira, Ivone; Calderón-Franco, David; Gorecki, Adrian; Purkrtova, Sabina; Bartacek, Jan; Dziewit, Lukasz; Singleton, Caitlin M.; Nielsen, Per Halkjær; Weissbrodt, David Gregory; Manaia, Célia M.Antibiotic resistance (AR) is a global problem requiring international cooperation and coordinated action. Global monitoring must rely on methods available and comparable across nations to quantify AR occurrence and identify sources and reservoirs, as well as paths of AR dissemination. Numerous analytical tools that are gaining relevance in microbiology, have the potential to be applied to AR research. This review summarizes the state of the art of AR monitoring methods, considering distinct needs, objectives and available resources. Based on the overview of distinct approaches that are used or can be adapted to monitor AR, it is discussed the potential to establish reliable and useful monitoring schemes that can be implemented in distinct contexts. This discussion places the environmental monitoring within the One-Health approach, where two types of risk, dissemination across distinct environmental compartments, and transmission to humans, must be considered. The plethora of methodological approaches to monitor AR and the variable features of the monitored sites challenge the capacity of the scientific community and policy makers to reach a common understanding. However, the dialogue between different methods and the production of action-oriented data is a priority. The review aims to warm up this discussion.