Browsing by Author "Ferreira, Isabel"
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- Production of histamine and tyramine by bacteria isolated from Portuguese vacuum-packed cold-smoked fishPublication . Silva, Manuela V. da; Pinho, Olívia; Ferreira, Isabel; Plestilov, LindaAn agar medium containing histidine or tyrosine incubated anaerobically was used for detecting the decarboxylating activity of bacteria isolated from Portuguese vacuum-packed cold-smoked fish during chilled storage. The capacity of each bacterial isolate to produce histamine and tyramine was studied at 25 and 5 C incubated for 48 h and 10 days, respectively. More strains produced histamine and tyramine at 25 C compared with 5 C although lactic acid bacteria (LAB) strains exhibited similar results at the two different temperatures. Tyramine was produced by majority of the isolates tested although very low concentrations were produced at 5 C as confirmed by high-pressure liquid chromatography (HPLC). Tyrosine-agar was shown to be a good indicator medium for detection of bacteria that produced high levels of tyramine, since typical colonies surrounded by a translucent halo were easily recognised. LAB identified as Lc. Lactis lactis 1 and Carnobacterium divergens were detected as tyramine-producing bacteria. Acinetobacter spp. and Pseudomonas spp., isolated from all Portuguese smoked fish products, were negative on histidine-agar, but HPLC identified considerable quantities of histamine produced in a broth medium.
- Screening of the antimicrobial activity of wild mushrooms phenolic extracts against clinical isolatesPublication . Alves, Maria; Ferreira, Isabel; Martins, Anabela; Pintado, ManuelaAntimicrobial drugs have long been used for prophylactic and therapeutic purposes; however the drug-resistant bacterial strains have been creating serious treatment problems. This situation has forced the search of new antimicrobial substances effective against pathogenic microorganisms resistant to conventional treatments. Natural resources have been tested and among them mushrooms could be an alternative. This work aimed to screen the antimicrobial activity of phenolic extracts of 13 edible mushroom species, collected in Bragança, against several clinical isolates obtained in Hospital Center of Trás-os-Montes and Alto Douro, Portugal. Microdilution method was used to determine the Minimum Inhibitory Concentration (MIC) and the Minimum Bactericidal Concentration (MBC). MIC results showed that Russula delica and Fistula hepática extracts inhibited the growth of Gram negative (Escherichia coli, Pasteurella multocida, Morganella morganni, isolated from urine) and Gram positive (Staphylococcus aureus, MRSA- isolated from wound exudates, Enterococcus faecalis, Listeria monocytogenes- isolated from blood culture, Streptococcus agalactiae and Streptococcus pyogenes- isolated from vaginal swab)bacteria. A bactericide effect of both extracts was observed in Gram positive bacteria (L. monocytogenes, S. agalactiae and S. pyogenes); regarding Gram negative bacteria, a total inhibition effect was observed for P. multocida at the highest concentration tested (20 mg/ml). Lepista nuda extract exhibited a bactericide effect upon P. multocida at 5 mg/ml, and inhibited Proteus mirabilis (isolated from urine) at 20 mg/ml. Ramaria botrytus extract did not present activity against Gram-negative bacteria; nevertheless, it showed activity against E. faecalis , being bactericide for S. agalactiae and S. pyogenes. Moreover, this was the extract with the highest activity against L. monocytogenes with MBC of 10 mg/ml. Leucopaxillus giganteus extract inhibited the growth of E. coli and P. mirabilis, being bactericide for P. multocida, L. monocytogenes, S. pyogenes and S. agalactiae. Among all the studied mushrooms, Agaricus arvensis seemed to present the lowest antimicrobial activity against all the tested bacteria. The extracts are chemically characterized and the individual/combined phenolic compounds will be submitted to antimicrobial assays in order to identify compounds responsible for the mushrooms bioactivity.